Microplate Digestion of Minipreps from a Single Microcentrifuge Tube

摘要

There are numerous protocols of minipreparations of plasmid DNA to choose from. Recent reports improve the original alkaline lysis or boiling methods to reduce manipulation times. Many have reported rapid, cost-effective plasmid purification, but screening of the preparations is still labor-intensive. To further minimize time and cost, we have modified the procedure of Holmes and Quigley (i) to use a single microcentrifuge tube for both culturing and purifying the plas-mid DNA and (ii) to screen purified DNA by restriction enzyme analysis using a 96-well microplate.