ATCC technology assessment of Roche xCELLigence System: an electronic impedance-based cell sensing unit

摘要

This article describes our systematic investigation into the utility of employing electronic impedance-based cell sensing measurement systems to evaluate changes in cell behavior. We investigated changes resulting from routine passaging (data not shown) and intentional perturbation. Insights gathered from this and other studies present us with new opportunities for introducing advanced levels of cellular characterization and contribute towards development of superior cellular assays at ATCC. Changesin cellular morphology or growth rate can be early indicators of adverse cellular events. Detection and quantitation of these events with impedance-based cell sensing measurement systems appear to offer improved, real-time, label-free and non-invasive analysis of key cellular events. When adherent cells attach and spread on the sensor surface of an electrode, increases in impedance are recorded. Conversely, cells that round up or detach, even for a short time, cause impedance values to drop. One systemwell equipped to measure cell-impedance is the xCELLigence System from Roche Applied Science.