Somatic embryogenesis from broccoli stigmas in tissue culture

摘要

The factors regulating callus proliferation and hurt regeneration from stigma tissues are not sufficiently understood. To study the regenerative capacity of pistil elements, pistil of broccoli was cultured under simple culture conditions. Stigmas with style from broccoli pistils undergo somatic embryogenesis on Murashige and Skoog basal medium. Callus initiation occurred on basal medium supplemented with 4 fug center dot l(-1) BAP, 1.6 mg center dot l(-1) 2,4-D, 250 mg center dot l(-1) cascin hydrolysate and 30 g center dot l(-1) sucrose. Proembryo induction was observed after two callus subcultures. Calluses with globular embryos were cultured on basal medium with 2 mg center dot l(-1) BAP, 1 mg center dot l(-1) IAA and 40 g center dot l(-1) sucrose for development, maturation and germination of somatic embryos. A population of somatic embryos was maintained on medium containing 1 mg center dot l(-1) BAP and 2 mg center dot l(-1) NAA only. Adding NAA to MS medium containing BAP considerably enhanced root formation. After acclimatization, all plantlets developed well and produced phenotypically normal flowers.