Ability of Lactobacillus plantarum LS/07 to modify intestinal enzymes activity in chronic diseases prevention

摘要

The ability of probiotic strain Lactobacillus plantarum LS/07 to modify the activity of intestinal bacterial enzymes - beta-glucuronidase (beta-GLUCUR), beta-galactosidase (beta-GAL), and beta-glucosidase (beta-GLU) in prevention of chronic diseases - cancer, atherosclerosis and dysbiosis was investigated. The male Sprague-Dawley rats were randomly divided into 12 experimental groups: controls groups - C (control), AT (atherosclerotic), CC (carcinogenic), dysbiotic groups - each group in combination with antibiotics (ATB), probiotics groups - in combinatioan with probiotic (PRO) alone, and each group with combination of antibiotic and probiotic (ATB+PRO). In the control group the beta-glucuronidase activity did not change throughout the experiment. High fat diet in atherosclerotic group significantly increased the activity of beta-glucuronidase (P < 0.001) and beta-glucosidase (P < 0.01). Azoxymethane application in carcinogenic group significantly increased beta-glucuronidase (P < 0.01), but reduced beta-glucosidase (P < 0.01) activity. Daily application of probiotics alone and in combination with antibiotic increased beta-galactosidase, of beta-glucosidase, and decreased beta-glucuronidase activity. In control antibiotic group we observed significant increase in beta-glucuronidase (P < 0.05) and decreased beta-glucosidase (P < 0.01) activity which can be caused by the change of microflora in favor of coliform bacteria. These findings indicate the positive effects of probiotic Lactobacillus plantarum LS/07 and suggest its use in disease prevention in human medicine and some animal species.