Differentially Expressed Wound-response-related Proteins from a Major Agarwood-producing Tree, Aquilaria malaccensis Lam. Identified via 2-D Electrophoresis

摘要

Background: Wounding is the major trigger factor for agarwood formation in Aquilariamalaccensis. To date, the mechanism of agarwood formation in Aquilaria has been extensively exploredthrough anatomy and genomics approaches; however, at the protein level, it is yet to be reported.Objective: A comparative proteomics analysis was carried out to identify proteins responsive towounding in A. malaccensis, using two-dimensional gel electrophoresis (2-DE) coupled with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).Method: Proteins were extracted from A. malaccensis tree stems at 0, 6, 12, and 24 h after woundingand separated by 2-DE, sequenced using MALDI-TOF MS and identified through UniProt/Swiss-Protprotein database.Results: Changes in differentially displayed proteins were observed. A total of 15 protein spots wereidentified reproducible under 2-DE, of which only two protein spots showed differences in expressionaffected by the wounding treatment in a time-dependent experiment. They were predicted as MalateSynthase (MS) and the reduced Nicotinamide Adenine Dinucleotide Phosphate (NADPH) quinone oxidoreductasesubunit 2 B. Both proteins were directly and indirectly related to wounding treatments inplants and may be related to agarwood formation mechanism.Conclusion: As Aquilaria proteome is not available to date, this first report on differential protein expressionin response to wounding could serve as a useful information towards understanding agarwoodbiosynthesis at the protein level.