Rare Earth Doped Hydroxyapatite Nanoparticles for In Vitro Bioimaging Applications | Bentham Science

摘要

Background: Fluorescence based bioimaging is one of the widely used methodfor obtaining imperative information on life processes.Objective: Within the expansive spectrum of fluorescent agents being investigated, the trivalentLanthanide (Ln) ion based nanoparticles have attracted attention due to their intrinsicluminescence property.Methods: Here we report a modified sol gel assisted synthesis of Europium (Eu) and Samarium(Sm) doped Hydroxyapatite nanoparticles (HAp NPs). Doping Ln ions in the selffluorescenthydroxyapatite lattice contributed towards an increased luminescence in theNPs.Results: The XRD patterns reveal that the Eu+3 and Sm+3 doped HAp NPs display thecharacteristic peaks of hydroxyapatite in a hexagonal lattice structure, and the FTIR dataconfirms presence of characteristic functional groups. The as-synthesized HAp NPs exhibitshort rod-shaped morphology with average length less than 60 nm. Upon excitation at representativewavelengths, the doped HAp NPs demonstrated characteristic emission lines ofEu+3 and Sm+3.Conclusion: The as-synthesized NPs displayed no toxicity towards HeLa cells and areeasily internalized, exhibiting their potential as promising live cell bioimaging agents.