Evaluation of Estrogen Receptor a and B Genes Expression in Normal and Neoplastic Mammary Gland in Dogs by Real-time PCR

摘要

Background: Estrogen and progesterone play a key role in mammary tumori-genesis of female dogs. Malignant tumors are considered hormone-dependent neoplasms, which may reappear after surgical removal or might spread to other organs such as liver, lymph nodes, and lungs. Objective: The purpose of this study was to evaluate the expression of estrogen receptor a and B genes in normal and neoplastic mammary glands of female dogs. Methods: Ten neoplastic tumors (benign and malignant) and 10 normal samples were selected. After RNA extraction and cDNA synthesis, ERa and ERB genes were amplified by Real-time PCR based on SYBR green related to B-actin reference gene. Results: The obtained Ct and ACt values were analyzed by GLM procedure of SAS software v9.2. The results showed that the expression of ERa and ERB genes in neoplastic cases was over- and down-expressed respectively as compared with normal samples (p < 0.01).