A mosaic structure multi-level vascular network design for skull tissue engineering

摘要

In human skull tissue engineering scaffolds, cell growth and osteogenesis are limited due to the lack of vascular structure. Therefore, a mosaic structure vascular parameterized design method is proposed according to the scanning characteristics of the diploic vein. Using micro-CT scans of skull samples, the features of the diploic vein were extracted, and a multi-level vascular network model was established based on a power diagram. Considering the characteristics of blood flow in the veins, finite element analysis (FEA) of the fluid-solid coupling was established to analyze the effect of blood on vessels with four-level mosaic structures. The results showed that the deformation and stress distribution of vessels were reasonable, and the blood pressure, velocity and shear stress in the designed vascular structure could meet the cell growth requirements. The mosaic structure was prepared by PDMS and cultured in vitro using HUVECs. It was found that most of the cells survived after 48 h, and some cells were attached to the surface mosaic structure. In this method, different levels of vessels nest together, with a curvature that matches the shape of the skull, forming a similar morphology to the native diploic vein, and the local structures can be adjusted flexibly. This mosaic structure vascular design method can be used for network vascular design and experimental studies in hard tissues.