Synthesized glucocorticoid‐induced leucine zipper peptide inhibits photoreceptor apoptosis and protects retinal function in light‐induced retinal degeneration model

摘要

Abstract Background This study aimed to investigate the neuroprotective function of a synthesized glucocorticoid‐induced leucine zipper peptide (GILZ‐p) in a light‐induced retinal degeneration model. Methods The GILZ 98‐134 peptide was synthesized and injected intravitreally into Sprague Dawley rats. Retinal injury was then induced in the rats by exposing their eyes to constant white light (5000?lux) for 24?h. The activation of retinal caspases‐9/3 and the release of cytochrome c from the mitochondria to the cytosol were measured at 1, 3, 5 and 7 d after light injury. Photoreceptor apoptosis was evaluated with terminal‐deoxynucleotidyl‐transferase‐mediated deoxyuridine triphosphate‐biotin nick end labelling (TUNEL) staining at 3 d after injury. Haematoxylin and eosin staining and electroretinography were used to observe the changes in the retinal morphology and function, respectively, at 7 and 14?d after light injury. Results The intravitreally injected synthesized GILZ‐p successfully penetrated to the retina and significantly inhibited the activation of retinal caspase‐3 and caspase‐9 at 1, 3, 5 and 7 d after light injury, and reduced the number of TUNEL‐positive photoreceptors at 3 d after light injury. GILZ‐p pre‐treatment also alleviated cytochrome c release and rescued mitochondria‐mediated apoptosis after injury. Simultaneously, GILZ‐p pre‐treatment also mitigated the light‐induced thinning of the outer nuclear layer and the loss of retinal function at 7 and 14?d after light injury, respectively. Conclusions The synthesized GILZ‐p prevented light‐induced photoreceptor apoptosis and protected retinal function from degeneration, and is therefore a potential therapeutic option for degenerative retinal diseases.