Identification and molecular characterization of cytochrome P450 (CYP450) family genes in the marine ciliate Euplotes crassus: The effect of benzo [a] pyrene and beta-naphthoflavone

摘要

Marine ciliate Euplotes crassus, a single-cell eukaryote, and has been considered as a model organism for monitoring of environmental pollutions in sediments. Cytochrome P450 (CYP450) monooxygenase are phase I enzyme involved in detoxification of environmental pollutants, such as polycyclic aromatic hydrocarbons (PAHs). However, little information on CYP450 family genes in ciliate is available. In the present study, acute toxicity of PAH, benzo[a]pyrene (B[a]P) and PAH-like model compound, beta-naphthofiavone (beta-NF), was investigated; full-length cDNA sequences and genomic structure of five CYP450 genes (CYP5680A1, CY135681A1, CYP5681B1, CYP5682A1, and CYP5683A1) were analyzed; and finally their activities and transcriptional changes were measured after exposure to PAHs for 48 h. According to the results, B[a]P, exposure showed a negative effect on E. crassus survival, whereas beta-NF exposure showed no significant effect. The 8 h-LC50 value of B[a]P was determined to be 2.449 mu M (95%-C.L., 7.726-3.619 mu M). Five genes belonging to the CYP450 family had conserved domains and clustered with those of ciliate group, as revealed in phylogenetic analysis. CYP activity did not change after exposure to B[a]P, whereas it was slightly, but significantly, induced after exposure to beta-NF. The mRNA expression of five CYP450 genes was significantly modulated in a concentration- and time dependent manner after exposure to both the chemicals. Our findings suggest that CYP450 genes in E. crassus may be involved in detoxification of B[a]P and beta-NF. This study would give a better understanding about the mode of action of B[a]P and beta-NF in marine ciliates at the molecular level.