Simultaneous Electrochemical Evaluation of Ascorbic Acid, Epinephrine and Uric Acid at Disposable Pencil Graphite Electrode: Highly Sensitive Determination in Pharmaceuticals and Biological Liquids by Differential Pulse Voltammetry

摘要

Aim and Objective: As is known, AA, EP and UA can also coexist in biological fluids.Therefore, the determination of the levels of these compounds in biological fluids is extremelyimportant both for the diagnosis and treatment of the related diseases. In the presence of manyinterfering substances in biological fluids such as blood and urine samples, it is very important thatthese compounds can be selectively analyzed.Materials and Methods: All electrochemical experiments were performed using an AutolabPGSTAT 128N potentiostat. Before beginning the electrochemical measurements, the PGE wasactivated. The electrochemical pretreatment of PG was exercised by anodically +1.40 V for 60 s.Then, measurements were performed with CV (-0.4 V to 1.2 V) and DPV (-0.2 V to 0.7 V) for singleand simultaneous voltammetric behaviour of AA, EP, and UA in the electrochemical method.Results: The anodic peak potentials of AA and UA were observed at about +0.32 V and +0.62 V,respectively. On the other hand, for EP, while anodic peak potential was observed at about +0.53 V,in the reverse scan, cathodic peak potentials were observed at about +0.41 V and +0.007 V. Thereduction peak observed at +0.3 V with the oxidation peak observed at +0.53 V are the reversiblepeaks. In the method developed for the electrochemical simultaneous determination of AA, EP andUA using PGE with DPV technique in BR buffer solution (pH 4.0), the anodic peak potentials aresufficiently separated from each other.Conclusion: A voltammetric method was developed for the simultaneous determination of AA, EPand UA with PGE for the first time. Here, the most important thing is that the simultaneousdetermination of AA, EP and UA was successfully achieved with that targeted voltammetric methodwhich was sensitive, low-cost, practical and well-repeated; and that these were proven to beselectively applicable in pharmaceutical products and biological liquids.