Cloning of the genes from Erwinia herbicola EH252 necessary for production of an antibacterial compound which inhibits Erwinia amylovora

摘要

E. herbicola Eh252 reduces the incidence of fire blight on apple and pear blossoms by producing an antibacterial compound which reduces growth of E. amylovora. Mutants which do not produce this substance are called Ant-. The genes necessary for theproduction of this substance were cloned and transferred into Escherichia coli yielding 5 clones that inhibited growth of E. amylovora. Plasmids isolated from the clones restored antibiotic ability to Ant- mutants. Two plasmids were used to complement 10