Quantification of 3-Monochloropropane-1,2-diol Esters in Edible Oils by Large-Volume Injection Coupled to Comprehensive Gas Chromatography-Time-of-Flight Mass Spectrometry

摘要

3-Monochloropropane-1,2-diol (3-MCPD) esters are a group of process contaminants formed during the refining of edible oils and fats. A method for the determination of 3-MCPD esters in such oils and fats based on large-volume injection-comprehensive gas chromatography-time-of-flight mass spectrometry (LVI-GC x GC-ToF MS) is presented. The simplified method for sample preparation consists of alkaline hydrolysis followed by extraction of 3-MCPD from the lipid matrix and derivatization using phenylboronic acid. The limit of detection was 0.8 mu g kg(-1), reported as free 3-MCPD. The repeatability was better than 2.7% relative standard deviation for levels around 0.5 mu g g(-1) 3-MCPD. The GC x GC-ToF MS system was shown to be stable over 100 analyses.