Development and validation of a stability-indicating LC method for simultaneous analysis of aceclofenac and paracetamol in conventional tablets and in microsphere formulations

摘要

A stability-indicating reversed-phase LC method for analysis of aceclofenac and paracetamol in tablets and in microsphere formulations has been developed and validated. The mobile phase was 80:20 (v/v) methanol-phosphate buffer (10 mM at pH 2.5 +/- 0.02). UV detection was at 276 nm. The method was linear over the concentration ranges 16-24 and 80-120 mu g mL(-1) for aceclofenac and paracetamol, respectively, with recovery in the range 100.9-102.22%. The limits of detection and quantitation for ACF were 0.0369 and 0.1120 mu g mL(-1), respectively; those for PCM were 0.0631 and 0.1911 mu g mL(-1), respectively.