Post‐translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase

Sutherland Ashley R.; Alam Md. Kausar; Geyer C. Ronald

摘要

Abstract >Exploiting the innate modularity of proteins has allowed advances across the fields of synthetic biology and biotechnology. By using standardized protein components as building blocks, complex, multiprotein assemblies with sophisticated functions can be generated; feats previously not possible with strictly genetic‐engineering approaches. The development of strategies for protein assembly is accelerating, pushing the boundaries of protein architecture. SpyTag and SpyCatcher protein ligase is a recent advance in this field that allows plug‐and‐play modularity by harnessing post‐translational protein assembly. Herein, we review the latest applications of this powerful tool including novel enzyme assemblies, modularizing protein display, and the generation of antibody and antibody‐like “devices” by using SpyTag/SpyCatcher technology. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >利用蛋白质的先天模块化允许穿过合成生物学和生物技术领域的进步。通过使用标准化的蛋白质组分作为构建块，可以产生复杂的具有复杂功能的多曲线组件; 以前不可能拥有严格的遗传工程方法以前不可能。蛋白质组件策略的发展正在加速，推动蛋白质结构的界限。 Spytag和脱磷酸蛋白蛋白连接酶是该领域最近的前进，其通过利用翻译后蛋白质组件来允许即插即用的模块化。在此，我们通过使用SpyTag / Spycatcher技术，审查包括新型酶组件，模块化蛋白质显示器，模块化蛋白质显示器和产生抗体和抗体的“装置”的最新应用。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology 》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b> <span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sutherland Ashley R.&option=202" target="_blank" rel="nofollow">Sutherland Ashley R.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alam Md. Kausar&option=202" target="_blank" rel="nofollow">Alam Md. Kausar;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Geyer C. Ronald&option=202" target="_blank" rel="nofollow">Geyer C. Ronald;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of BiochemistryUniversity of SaskatchewanSaskatoon SK S7N 5E5 Canada;</p> <p>Donnelly Centre for Cellular and Biomolecular ResearchUniversity of TorontoToronto ON M5S3E1 Canada;</p> <p>Department of Pathology and Laboratory MedicineUniversity of SaskatchewanSaskatoon SK S7N 5E5 Canada;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=post-translational protein assembly&option=203" rel="nofollow">post-translational protein assembly;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=protein engineering&option=203" rel="nofollow">protein engineering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=SpyTag/SpyCatcher&option=203" rel="nofollow">SpyTag/SpyCatcher;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=synthetic biology&option=203" rel="nofollow">synthetic biology;</a> </p> <div class="translation"> 机译：翻译后蛋白质组件;蛋白质工程;SPYTAG / SPYCATCHER;合成生物学; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022056500.html">Post‐translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sutherland Ashley R.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sutherland Ashley R.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alam Md. Kausar&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Alam Md. Kausar,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Geyer C. Ronald&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Geyer C. Ronald </a> <a href="/journal-foreign-16039/" target="_blank" rel="nofollow" class="tuijian_authcolor">Chembiochem: A European journal of chemical biology .</a> <span>2019</span> <span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过使用SpyTag / Spycatcher蛋白质连接酶将蛋白质部件的翻译成组装成复杂的装置</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022055577.html">Synthetic Modular Antibody Construction by Using the SpyTag/SpyCatcher Protein‐Ligase System</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alam Md Kausar&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Alam Md Kausar,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gonzalez Carolina&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gonzalez Carolina,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hill Wayne&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hill Wayne,</a> <a href="/journal-foreign-16039/" target="_blank" rel="nofollow" class="tuijian_authcolor">Chembiochem: A European journal of chemical biology .</a> <span>2017</span> <span>,第22期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用SpyTag / Spycatcher蛋白质连接酶系统合成模块化抗体构造</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704010182544.html">Interaction proteins of invertase and invertase inhibitor in cold-stored potato tubers suggested a protein complex underlying post-translational regulation of invertase</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yuan Lin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yuan Lin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jun Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jun Liu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xun Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xun Liu,</a> <a href="/journal-foreign-29433/" target="_blank" rel="nofollow" class="tuijian_authcolor">Plant Physiology and Biochemistry .</a> <span>2013</span> <span>,第Null期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：冷藏马铃薯块茎中转化酶和转化酶抑制剂的相互作用蛋白表明，蛋白酶在翻译后调控中具有潜在的蛋白质复合体</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-257539_thesis/0705010727770.html">An Iterative Search Algorithm for Protein Identification from Complex Patterns of Post-translational Modifications</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xin Huang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xin Huang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Huang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lin Huang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hong Peng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hong Peng,</a> <a href="/conference-foreign-257539/" target="_blank" rel="nofollow" class="tuijian_authcolor">American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics .</a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从翻译后修改复杂模式的蛋白质识别迭代搜索算法</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/0206191411.html">Cell-free protein synthesis of complex proteins and protein assemblies containing post-translational modification.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Goerke, Aaron Rudy.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Goerke, Aaron Rudy. </a> <span>2008</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：复杂蛋白质和包含翻译后修饰的蛋白质装配体的无细胞蛋白质合成。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040003365774.html">A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Md. Kausar Alam&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Md. Kausar Alam,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Michelle Brabant&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Michelle Brabant,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Raja Solomon Viswas&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Raja Solomon Viswas,</a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于SpyTag / SpyCatcher蛋白连接酶系统的新型合成三价单链可变片段（tri-scFv）构建平台</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000124428863.html">A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Md. Kausar Alam&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Md. Kausar Alam,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Michelle Brabant&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Michelle Brabant,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Raja Solomon Viswas&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Raja Solomon Viswas,</a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：一种新型合成三价单链可变片段（三SCFV）构建平台，基于Spytag / Spycatcher蛋白连接酶系统</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/ntis-science-report_ad_thesis/02071117607.html">Assembly of Photosynthetic Antenna Protein Complexes from Algae for Development of Nano-biodevice and Its Fuelization.</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nango, M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nango, M. </a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：藻类光合天线蛋白复合物的组装，用于纳米生物装置的发展及其燃料化。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-liaoning-normal-university-natural-science-edition_thesis/0201270222381.html">GPI锚——一种复杂的蛋白质翻译后修饰</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李庆伟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李庆伟</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=宋涛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,宋涛</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=勾萌&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,勾萌</a> <span> <a href="/journal-cn-4664/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 辽宁师范大学学报（自然科学版） </a> </span> <span> . 2019</span><span> ,第002期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_life-science-instruments_thesis/0201225140733.html">非聚丙烯酰胺凝胶电泳(Non-PAGE)的复杂蛋白质组表达分析:一种新的二维液相技术用于完整蛋白质的高通量分离分析</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tim Barder&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Tim Barder</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李少伟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李少伟</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=周仲驹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,周仲驹</a> <span> <a href="/journal-cn-8255/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生命科学仪器 </a> </span> <span> . 2004</span><span> ,第002期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_china-animal-husbandry-veterinary-medicine_thesis/0201270687862.html">蛋白质组学及蛋白质翻译后修饰在畜牧领域中的应用研究进展</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王欣悦&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王欣悦</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张莉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张莉</a> <span> <a href="/journal-cn-7153/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国畜牧兽医 </a> </span> <span> . 2019</span><span> ,第004期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-academic-journal-abstracts_thesis/020121144388.html">生物化学——蛋白质巯基亚硝基化——一种典型氧化还原依赖的蛋白质翻译后修饰</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈畅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈畅</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄波&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,黄波</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=韩佩韦&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,韩佩韦</a> <span> <a href="/journal-cn-5250/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国学术期刊文摘 </a> </span> <span> . 2006</span><span> ,第021期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201296578213.html">蛋白质的翻译后加工3．蛋白质定位在细胞内的信号</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王克夷&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王克夷</a> <span> <a href="/journal-cn-57125/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生命的化学 </a> </span> <span> . 1995</span><span> ,第3期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-8934_thesis/020222236342.html">蛋白质组学技术在中药复杂体系研究中的应用</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=岳庆喜&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 岳庆喜</a> <span> <a href="/conference-cn-8934/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2012生物产业发展与文化·民生——全国博士后学术论坛 </a> <span> <span> . 2012</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314657468.html">使用多种基于序列的特征预测蛋白质的翻译后修饰位点</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈庆云&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈庆云</a> <span> . 2017</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120104840938.html">具有催泪成分合成酶活性的蛋白质或多肽、编码该蛋白质或多肽的 DNA、利用该 DNA制造具有催泪成分合成酶活性的蛋白质或多肽的制造方法以及具有抑制该蛋白质或多肽的mRNA翻译的功能的核酸分子</a> <b>[P]</b> . <span> 中国专利： CN1639337A </span> <span> . 2005-07-13</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120100344694.html">肝型脂肪酸结合蛋白质制剂、对其评价的方法、抑制使用其的测定中的肝型脂肪酸结合蛋白质引起的测定值的变动幅度的方法、肝型脂肪酸结合蛋白质、其的制造方法、编码其的DNA、由该DNA转化得到的细胞、制作肝型脂肪酸结合蛋白质的校准曲线的方法及对该蛋白质进行定量的方法</a> <b>[P]</b> . <span> 中国专利： CN109121419A </span> <span> . 2019-01-01</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130415863931.html">Methods and Compositions for Detecting, Imaging, and Treating Small Cell Lung Cancer Utilizing Post-Translationally Modified Residues and Higher Molecular Weight Antigenic Complexes in Proteins</a> <b>[P]</b> . <span> 外国专利：  US2015232539A1 </span> <span> . 2015-08-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：利用翻译后修饰的残基和蛋白质中较高分子量的抗原复合物检测，成像和治疗小细胞肺癌的方法和组合物 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130417297485.html">METHODS AND COMPOSITIONS FOR DETECTING, IMAGING, AND TREATING SMALL CELL LUNG CANCER UTILIZING POST-TRANSLATIONALLY MODIFIED RESIDUES AND HIGHER MOLECULAR WEIGHT ANTIGENIC COMPLEXES IN PROTEINS</a> <b>[P]</b> . <span> 外国专利：  WO2014039733A1 </span> <span> . 2014-03-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：利用翻译后修饰的残留物和蛋白质中更高分子量的抗原复合物检测，成像和治疗小细胞肺癌的方法和成分 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130425730550.html">SELECTIVE ENRICHMENT OF POST-TRANSLATIONALLY MODIFIED PROTEINS AND/OR PEPTIDES FROM COMPLEX SAMPLES</a> <b>[P]</b> . <span> 外国专利：  US2010304493A1 </span> <span> . 2010-12-02</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：复杂样品中翻译后修饰蛋白和/或肽的选择性富集 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704022056500','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704022056500down" data-source="7," data-out-id="uQEWElnrv6fLPxR46uTl0iz9x3DfdDhRL7LGZ/3MqkwRUiVeE2seTwk38UqauFTC," data-f-source-id="7" data-title="Post‐translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="16039" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Post‐translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.9"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.9"></script> </body> <script> function kefu(customization) { var from = GetQueryString("from"); if (from == null || from == "") { from = "other"; } var url = getWxWorkQrCode(55, from, customization); if (url == "" || typeof url == 'undefined') { url = "https://wework.qpic.cn/wwpic/994838_0LUTS-VqR0quT1N_1675665967/0"; } tipDocGuidance(url, '人工辅助获取外文期刊材料'); } $(function () { var weiPuStatus = getCookie('WeiPuStatus'); if (weiPuStatus) { tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if ((sourcetype == 1 || sourcetype == 4) && inyn_provide_service_level != 4) { getJournal(); } }) </script> </html>