<i >BB        ‐factor Guided Proline Substitutions in        <i >Chromobacterium violaceumChromobacterium violaceum        Amine Transaminase: Evaluation of the Proline Rule as a Method for Enzyme Stabilization

Land Henrik; Campillo‐Brocal Jonatan C.; Svedendahl?Humble Maria; Berglund Per

摘要

Abstract > Biocatalysis is attracting interest in the chemical industry as a sustainable alternative in large‐scale chemical transformations. However, low operational stability of naturally evolved enzymes is a challenge and major efforts are required to engineer protein stability, usually by directed evolution. The development of methods for protein stabilization based on rational design is of great interest, as it would minimize the efforts needed to generate stable enzymes. Here we present a rational design strategy based on proline substitutions in flexible areas of the protein identified by analyzing B ‐factors. Several proline substitutions in the amine transaminase from Chromobacterium violaceum were shown to have a positive impact on stability with increased half‐life at 60?°C by a factor of 2.7 (variant K69P/D218P/K304P/R432P) as well as increased melting temperature by 8.3?°C (variant K167P). Finally, the presented method utilizing B ‐factor analysis in combination with the proline rule was deemed successful at increasing the stability of this enzyme. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >生物分析是吸引化学工业的兴趣作为大规模化学转化中的可持续替代品。然而，天然进化的酶的低运行稳定性是一种挑战，并且通常通过定向的进化来工程蛋白质稳定性所需的主要努力。基于理性设计的蛋白质稳定化方法的开发具有很大的兴趣，因为它将最小化产生稳定酶所需的努力。在这里，我们提出了一种基于通过分析蛋白质的柔性区域的脯氨酸取代的理性设计策略，所述蛋白质通过分析 B </ i>物质区鉴定。来自紫杉杆菌的胺转氨酶中的几种脯氨酸取代物被显示为稳定性对稳定性的稳定性影响，在60℃下增加半衰期为2.7（变体K69P / D218P / K304P / R432P））以及通过8.3℃（变体K167P）增加熔化温度。最后，利用 B </ i> - 因子分析与脯氨酸统治结合的呈现方法在增加该酶的稳定性时被认为是成功的。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology 》</a> <b style="margin: 0 2px;">|</b><span>2019年第10期</span><b style="margin: 0 2px;">|</b> <span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Land Henrik&option=202" target="_blank" rel="nofollow">Land Henrik;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Campillo‐Brocal Jonatan C.&option=202" target="_blank" rel="nofollow">Campillo‐Brocal Jonatan C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Svedendahl?Humble Maria&option=202" target="_blank" rel="nofollow">Svedendahl?Humble Maria;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Berglund Per&option=202" target="_blank" rel="nofollow">Berglund Per;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>KTH Royal Institute of TechnologyAlbaNova University Center106 91 Stockholm Sweden;</p> <p>KTH Royal Institute of TechnologyAlbaNova University Center106 91 Stockholm Sweden;</p> <p>Biovation ParkPharem Biotech ABForskargatan 20 J 151 36 S?dert?lje Sweden;</p> <p>KTH Royal Institute of TechnologyAlbaNova University Center106 91 Stockholm Sweden;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biocatalysis&option=203" rel="nofollow">biocatalysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme stabilization&option=203" rel="nofollow">enzyme stabilization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzymes&option=203" rel="nofollow">enzymes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=molecular modeling&option=203" rel="nofollow">molecular modeling;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=protein engineering&option=203" rel="nofollow">protein engineering;</a> </p> <div class="translation"> 机译：生物催化;酶稳定化;酶;分子造型;蛋白质工程; 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tuijian_authcolor">,袁春伟</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张震宇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张震宇</a> <span> <a href="/journal-cn-5081/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生物加工过程 </a> </span> <span> . 2014</span><span> ,第006期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-nanjing-university-mathematics-seminar_thesis/0201267503139.html">大肠杆菌二硫键异构酶DsbA和脯氨酸异构酶PPIaseA双顺反子表达载体的构建及其在大肠杆菌中的表达</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=华子春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 华子春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=许立&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,许立</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=孙爱龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,孙爱龙</a> <span> <a href="/journal-cn-51466/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 南京大学学报：数学半年刊 </a> </span> <span> . 1998</span><span> ,第002期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-navy-general-hospital_thesis/0201240272142.html">氯胺T法测定羟脯氨酸含量的方法改进</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=金博&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 金博</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李玉彬&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李玉彬</a> <span> <a href="/journal-cn-6372/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 海军总医院学报 </a> </span> <span> . 1995</span><span> ,第002期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_shaanxi-medical-laboratory_thesis/0201267498882.html">氯胺T法测定羟脯氨酸含量的方法改进</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=金博&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 金博</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李玉彬&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李玉彬</a> <span> <a href="/journal-cn-51494/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 陕西医学检验 </a> </span> <span> . 1994</span><span> ,第003期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-anatomy_thesis/0201222768099.html">N-乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸对基质金属蛋白酶-1和基质金属蛋白酶组织抑制因子-1的调节在大鼠矽肺纤维化形成中的作用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨方&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨方</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈萍&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈萍</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王俐玻&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王俐玻</a> <span> <a href="/journal-cn-5765/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 解剖学杂志 </a> </span> <span> . 2009</span><span> ,第006期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-21815_thesis/02022464114.html">沙利度胺对矽肺大鼠肺组织羟脯氨酸及FIZZ1表达的影响</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=辛建保&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 辛建保</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=彭清&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,彭清</a> <span> <a href="/conference-cn-21815/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十七次全国职业病学术交流会暨《中国工业医学杂志》创刊二十周年庆祝会、西南地区第十六届职业卫生与职业病学术交流会 </a> <span> <span> . 2007</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020316258317.html">多酶丙酮酸去除系统及其在ω-转氨酶不对称合成手性胺中的应用</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵妍淑&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 赵妍淑</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120103966792.html">表达活性D-脯氨酸还原酶的微生物以及生产活性D-脯氨酸还原酶的方法</a> <b>[P]</b> . <span> 中国专利： CN111032874A </span> <span> . 2020-04-17</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061202098447.html">脯氨酸羟化酶高活性自诱导表达方法及用其生产反式4-羟基-L-脯氨酸的转化方法</a> <b>[P]</b> . <span> 中国专利： CN103911355B </span> <span> . 2016.06.22</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130476677160.html">AMIDES OF N-SUBSTITUTED PROLINES, INHIBITORS OF AC ENZYME</a> <b>[P]</b> . <span> 外国专利：  IE812749L </span> <span> . 1982-05-24</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：N取代的脯氨酸的酰胺，AC酶的抑制剂 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130437682384.html">Compositions comprising lysine, proline, niacin and ascorbic acid and methods for lowering plasma lipoprotein(A) and risk factors of cardiovascular diseases</a> <b>[P]</b> . <span> 外国专利：  NZ525390A </span> <span> . 2006-04-28</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：包含赖氨酸，脯氨酸，烟酸和抗坏血酸的组合物以及降低血浆脂蛋白（A）和心血管疾病危险因素的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130412229263.html">methods for screening for a defective allele of a cofactor-administered enzyme-coding gene, for detecting a predisposition to a cofactor-dependent enzyme deficiency, for identifying and / or characterizing an enzyme deficiency within a metabolic pathway in a patient , to treat a metabolic enzyme deficiency in a patient, to screen for the risk of a condition or disease associated with aberrant foliate / homocysteine metabolism, to screen for potential chemotherapeutic response, and to screen for chemotherapeutic toxicity, to evaluate remediable enzyme deficiencies in a metabolic pathway, isolated nucleic acid, arrangement to detect a defective allele of a gene in the foliate / homocysteine metabolic pathway</a> <b>[P]</b> . <span> 外国专利：  BRPI0910097A2 </span> <span> . 2015-12-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：筛选辅因子给药的酶编码基因的缺陷等位基因，检测辅因子依赖性酶缺乏症的易感性，鉴定和/或表征患者代谢途径内酶缺乏症，治疗代谢的方法患者体内酶缺乏症，筛查与叶酸/同型半胱氨酸代谢异常有关的疾病或疾病的风险，筛查潜在的化疗反应，筛查化学疗法的毒性，评估代谢途径中可修复的酶缺乏症，分离的核酸酸，用于检测叶酸/同型半胱氨酸代谢途径中基因的缺陷等位基因 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704022056208','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img 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BB ‐factor Guided Proline Substitutions in Chromobacterium violaceumChromobacterium violaceum Amine Transaminase: Evaluation of the Proline Rule as a Method for Enzyme Stabilization

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