Immobilization of beta-galactosidase from Kluyveromyces lactis onto a polysiloxane-polyvinyl alcohol magnetic(mPOS-PVA)composite for lactose hydrolysis

摘要

beta-Galactosidase from Kluyveromyces lactis was covalently immobilized onto a mPOS-PVA,using glutaral-dehyde as activating agent and its properties were evaluated.The enzymatic water insoluble derivative displayed the same optimum pH(6.5)and optimum temperature(50°C)of the soluble enzyme.The apparent K_m~(app)and activation energy for both soluble and immobilized enzyme derivative were found to be not significantly different.The mPOS-PVA p-galactosidase preparation presented a higher operational and thermal stability than the soluble enzyme.This immobilized p-galactosidase also was effective in hydrolyzing lactose from milk.Hence,one can conclude that mPOS-PVA is an attractive and efficient support for p-galactosidase immobilization.