CELF1 preferentially binds to exon-intron boundary and regulates alternative splicing in HeLa cells

摘要

Abstract The current RIP-seq approach has been developed for the identification of genome-wide interaction between RNA binding protein (RBP) and the bound RNA transcripts, but still rarely for identifying its binding sites. In this study, we performed RIP-seq experiments in HeLa cells using a monoclonal antibody against CELF1. Mapping of the RIP-seq reads showed a biased distribution at the 3′UTR and intronic regions. A total of 15,285 and 1384 CELF1-specific sense and antisense peaks were identified using the ABLIRC software tool. Our bioinformatics analyses revealed that 5′ and 3′ splice site motifs and GU-rich motifs were highly enriched in the CELF1-bound peaks. Furthermore, transcriptome analyses revealed that alternative splicing was globally regulated by CELF1 in HeLa cells. For example, the inclusion of exon 16 of LMO7 gene, a marker gene of breast cancer, is positively regulated by CELF1. Taken together, we have shown that RIP-seq data can be used to decipher RBP binding sites and reveal an unexpected landscape of the genome-wide CELF1-RNA interactions in HeLa cells. In addition, we found that CELF1 globally regulates the alternative splicing by binding the exon-intron boundary in HeLa cells, which will deepen our understanding of the regulatory roles of CELF1 in the pre-mRNA splicing process. Highlights ? RIP-seq identifies the binding peaks and motifs of CELF1 protein on RNA targets in HeLa cells. ? 5′ and 3′ splice site motifs are highly enriched in the CELF1-bound peaks in HeLa cells. ? Transcriptome analysis reveals that alternative splicing is globally regulated by CELF1 in HeLa cells. ? The inclusion of exon 16 of LMO7 gene, a breast cancer signature gene, is positively regulated by CELF1.