Action of tannin on cellular membranes: Novel insights from concerted studies on lipid bilayers and native cells

摘要

Hydrolyzable tannin (3,6-bis-O-digalloyl-1,2,4-tri-O-galloyl-beta-D-glucose) has a dual effect on the cell membrane: (1) it binds to a plasmalemmal protein of the Chara corallina cell (C-50 = 2.7 +/- 0.3 NM) and (2) it forms ionic channels in the lipid membrane. Based on these facts, a molecular model for the interaction of tannins with the cell membrane is proposed. The model suggests that the molecules of hydrolyzable tannin bind electrostatically to the outer groups of the membrane protein responsible for the Ca2+-dependent chloride current and blocks it. Some tannin molecules penetrate into the hydrophobic region of the membrane, and when a particular concentration is reached, they form ion-conducting structures selective toward Cl-.