Molecular detection of phytoplasmas infecting apple trees in Poland

摘要

In 2010 more than 120 apple trees from different regions of Poland were tested for phytoplasma presence. Shoots from symptomatic and asymptomatic plants were collected and total DNA was extracted from phloem tissue. DNA was subjected to a nestedpolymerase chain reaction (PCR) for amplification of the 23S ribosomal RNA (rRNA) sequences using universal primers and primers specific for 'Candidatus Phytoplasma mali' and 'Ca. P. asteris'. Restriction fragment length polymorphism (RFLP) nucleotide sequence analysis of phytoplasmas' 16S-23S rDNA fragment analyses of PCR products made possible to identify 'Ca. P. mali' in seven apple trees and 'Ca. P. asteris' in two other trees.