High‐throughput lipidomics analysis to discover lipid biomarkers and profiles as potential targets for evaluating efficacy of Kai‐Xin‐San against APP/PS1 transgenic mice based on UPLC–Q/TOF–MS

摘要

Abstract Lipid metabolism has a significant function in the central nervous system and Alzheimer's disease (AD) is an age‐related senile disease characterized by central nerve degeneration. The pathological development of AD is closely related to lipid metabolism disorders. To reveal the influence of Kai‐Xin‐San (KXS) on lipid metabolism in APP/PSI transgenic mice and potential therapeutic targets for treating AD, brain tissue samples were collected and analyzed by high‐throughput lipidomics based on UPLC–Q/TOF‐MS. The collected raw data were processed by multivariate data analysis to discover the potential biomarkers and lipid metabolic profiles. Compared with the control wild‐type mouse group, nine potential lipid biomarkers were found in the AD model group, of which seven were up‐regulated and two were down‐regulated. Orally administrated KXS can reverse the changes in these potential biomarkers. Compared with the model group, a total of six differential metabolites showed a recovery trend and may be potential targets for KXS to treat AD. This study showed that high‐throughput lipidomics can be used to discover the perturbed pathways and lipid biomarkers as potential targets to reveal the therapeutic effects of KXS.