Ultra-low carbon dioxide partial pressure improves the galactosylation of a monoclonal antibody produced in Chinese hamster ovary cells in a bioreactor

Wang Chen; Wang Jiaqi; Chen Min; Fan Li; Zhao Liang; Tan Wen-Song

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Ultra-low carbon dioxide partial pressure improves the galactosylation of a monoclonal antibody produced in Chinese hamster ovary cells in a bioreactor

机译：超低二氧化碳部分压力改善了在生物反应器中的中国仓鼠卵巢细胞中产生的单克隆抗体的半乳糖组合

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摘要

To explore the influence of ultra-low carbon dioxide partial pressure (pCO(2)) on the monoclonal antibody (mAb) N-glycosylation profile in Chinese hamster ovary (CHO) cell culture.

机译：探讨超低二氧化碳分压（PCO（2））对中国仓鼠卵巢（CHO）细胞培养中单克隆抗体（MAB）N-糖基化谱的影响。

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来源
《Biotechnology Letters》 |2018年第8期|共8页
作者
Wang Chen; Wang Jiaqi; Chen Min; Fan Li; Zhao Liang; Tan Wen-Song;
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作者单位

East China Univ Sci &

Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

East China Univ Sci &

Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

Shanghai Bioengine Biotechnol Co Ltd Shanghai 200237 Peoples R China;

East China Univ Sci &

Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

East China Univ Sci &

Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

East China Univ Sci &

Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
Chinese hamster ovary cell; Glycosylation; pCO(2); Scale-up; Monoclonal antibody;

机译：中国仓鼠卵巢细胞;糖基化;PCO（2）;Scale-up;单克隆抗体;

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专利

1. hsBLyS基因在中国仓鼠卵巢细胞中的表达及其在免疫小鼠中诱发的抗体反应 [J] . 吴海涛, 吴玉蓉, 胡云龙, 动物学报（英文版） . 2006,第001期
2. Ultra-low carbon dioxide partial pressure improves the galactosylation of a monoclonal antibody produced in Chinese hamster ovary cells in a bioreactor [J] . Wang Chen, Wang Jiaqi, Chen Min, Biotechnology Letters . 2018,第8期

机译：超低二氧化碳部分压力改善了在生物反应器中的中国仓鼠卵巢细胞中产生的单克隆抗体的半乳糖组合
3. Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture [J] . Masayoshi Onitsuka, Akira Kawaguchi, Ryutaro Asano Journal of Bioscience and Bioengineering . 2014,第5期

机译：中国仓鼠卵巢细胞在生物反应器培养物中产生的聚集抗体的糖基化分析
4. Efficient enrichment of high-producing recombinant Chinese hamster ovary cells for monoclonal antibody by flow cytometry [J] . Okumura Takeshi, Masuda Kenji, Watanabe Kazuhiko, Journal of Bioscience and Bioengineering . 2015,第3期

机译：流式细胞术有效富集高产重组中国仓鼠卵巢细胞的单克隆抗体
5. Flow cytometry screening strategy for the enrichment of high-producing Chinese Hamster ovary cells for monoclonal antibody manufacturing [C] . Takeshi Okumura Cell culture engineering XV . 2016

机译：流式细胞仪筛选策略，用于富集高产的中国仓鼠卵巢细胞以生产单克隆抗体
6. Understanding Transcriptional regulation in recombinant monoclonal producing Chinese hamster ovary cells. [D] . Dahodwala, Hussain. 2011

机译：了解重组产生单克隆中国仓鼠卵巢细胞的转录调控。
7. Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line [O] . Fatemeh Torkashvand, Fereidoun Mahboudi, Manouchehr Vossoughi, 2018

机译：产生单克隆抗体的中国仓鼠卵巢细胞系中对设计氨基酸进料的细胞反应的定量蛋白质组学分析
8. Ouantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line [O] . Fatemeh Torkashvand, Fereidoun Mahboudi, Manouchehr Vossoughi, 2018

机译：在生产中国仓鼠卵巢细胞的单克隆抗体饲料中对设计氨基酸饲料的细胞反应的uhantative蛋白质组学分析

Ultra-low carbon dioxide partial pressure improves the galactosylation of a monoclonal antibody produced in Chinese hamster ovary cells in a bioreactor

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