• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology and Applied Biochemistry >Analysis of the efficiency of recombinant Escherichia?coliEscherichia?coli strain cultivation in a gas‐vortex bioreactor
【24h】

Analysis of the efficiency of recombinant Escherichia?coliEscherichia?coli strain cultivation in a gas‐vortex bioreactor

机译：重组大肠杆菌的效率分析http://www.wiley.com/namespaces/wiley“coli 大肠杆菌？在气体涡流生物反应器中的Coli菌株培养

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract > The levels of aeration and mass transfer are critical parameters required for an efficient aerobic bioprocess, and directly depend on the design features of exploited bioreactors. A novel apparatus, using gas vortex for aeration and mass transfer processes, was constructed in the Center of Vortex Technologies (Novosibirsk, Russia). In this paper, we compared the efficiency of recombinant Escherichia coli strain cultivation using novel gas‐vortex technology with conventional bioprocess technologies such as shake flasks and bioreactors with mechanical stirrers. We demonstrated that the system of aeration and agitation used in gas‐vortex bioreactors provides 3.6 times higher volumetric oxygen transfer coefficient in comparison with mechanical bioreactor. The use of gas‐vortex bioreactor for recombinant E.?coli strain cultivation allows to increase the efficiency of target protein expression at 2.2 times for BL21(DE3)/pFK2 strain and at 3.5 times for auxotrophic C600/pRT strain (in comparison with stirred bioreactor). </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >级别通气和传质是有效的有氧生物过程所需的关键参数，直接取决于利用生物反应器的设计特征。使用气体涡流进行通气和传质工艺的新颖设备，是在涡旋技术（Novosibirsk，俄罗斯）的中心构建的。在本文中，我们将重组大肠杆菌的效率与具有常规生物过程技术的新型气涡技术进行了测量培养的效率，例如具有机械搅拌器的摇瓶和生物反应器。我们证明，与机械生物反应器相比，气体涡流生物反应器中使用的曝气和搅拌系统提供了3.6倍的体积氧传输系数。用于重组的气体 - 涡旋生物反应器 E.?COLI </ i>菌株培养允许在BL21（DE3）/ PFK2菌株的2.2倍下增加目标蛋白表达的效率，并且在3.5倍的营养营养C600 / PRT菌株（与搅拌生物反应器相比）。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2017年第5期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Savelyeva Anna V.&option=202" target="_blank" rel="nofollow">Savelyeva Anna V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nemudraya Anna A.&option=202" target="_blank" rel="nofollow">Nemudraya Anna A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Podgornyi Vladimir F.&option=202" target="_blank" rel="nofollow">Podgornyi Vladimir F.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Laburkina Nadezhda V.&option=202" target="_blank" rel="nofollow">Laburkina Nadezhda V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ramazanov Yuriy A.&option=202" target="_blank" rel="nofollow">Ramazanov Yuriy A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Repkov Andrey P.&option=202" target="_blank" rel="nofollow">Repkov Andrey P.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kuligina Elena V.&option=202" target="_blank" rel="nofollow">Kuligina Elena V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Richter Vladimir A.&option=202" target="_blank" rel="nofollow">Richter Vladimir A.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Laboratory of BiotechnologyInstitute of Chemical Biology and Fundamental MedicineNovosibirsk Russia;</p> <p>Laboratory of BiotechnologyInstitute of Chemical Biology and Fundamental MedicineNovosibirsk Russia;</p> <p>Laboratory of BiotechnologyInstitute of Chemical Biology and Fundamental MedicineNovosibirsk Russia;</p> <p>Laboratory of BiotechnologyInstitute of Chemical Biology and Fundamental MedicineNovosibirsk Russia;</p> <p>JSC “The Center of Vortex Technologies ”Koltsovo Russia;</p> <p>JSC “The Center of Vortex Technologies ”Koltsovo Russia;</p> <p>Laboratory of BiotechnologyInstitute of Chemical Biology and Fundamental MedicineNovosibirsk Russia;</p> <p>Laboratory of BiotechnologyInstitute of Chemical Biology and Fundamental MedicineNovosibirsk Russia;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioprocess optimization&option=203" rel="nofollow">bioprocess optimization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Escherichia coli cultivation&option=203" rel="nofollow">Escherichia coli cultivation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gas‐vortex bioreactor&option=203" rel="nofollow">gas‐vortex bioreactor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mechanical bioreactor&option=203" rel="nofollow">mechanical bioreactor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=volumetric oxygen transfer coefficient&option=203" rel="nofollow">volumetric oxygen transfer coefficient;</a> </p> <div class="translation"> 机译：生物过程优化;大肠杆菌栽培;气体涡流生物反应器;机械生物反应器;体积氧转印系数; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881123.html">Analysis of the efficiency of recombinant <i xmlns="http://www.wiley.com/namespaces/wiley">Escherichia?coliEscherichia?coli strain cultivation in a gas‐vortex bioreactor</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Savelyeva Anna V.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Savelyeva Anna V.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nemudraya Anna A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nemudraya Anna A.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Podgornyi Vladimir F.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Podgornyi Vladimir F.,</a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2017</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：重组<i XMLNS =“http://www.wiley.com/namespaces/wiley”>大肠杆菌的效率分析http://www.wiley.com/namespaces/wiley“coli </ i>大肠杆菌？在气体涡流生物反应器中的Coli菌株培养</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028288089.html">Enhancement of? <sc xmlns="http://www.wiley.com/namespaces/wiley">l</sc>l ‐phenylalanine production in <i xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coliEscherichia coli by heterologous expression of <i xmlns="http://www.wiley.com/namespaces/wiley">VitreoscillaVitreoscilla hemoglobin</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Wei‐Bin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wu Wei‐Bin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guo Xiao‐Lei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guo Xiao‐Lei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Ming‐Liang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Ming‐Liang,</a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2018</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：增强？ <sc xmlns =“http://www.wiley.com/namespaces/wiley”> l </ sc> l -phenylanine生产在<i xmlns =“http://www.wiley.com/namespaces/wiley”>中 Escherichia coli </ i>通过异源表达的<i xmlns =“http://www.wiley.com/namespaces/wiley”> vietreoscilla </ i> Vitreoscilla血红蛋白</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881205.html">Enhancement of? <sc xmlns="http://www.wiley.com/namespaces/wiley">l</sc>l ‐phenylalanine production in <i xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coliEscherichia coli by heterologous expression of <i xmlns="http://www.wiley.com/namespaces/wiley">VitreoscillaVitreoscilla hemoglobin</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Wei‐Bin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wu Wei‐Bin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guo Xiao‐Lei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guo Xiao‐Lei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Ming‐Liang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Ming‐Liang,</a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2018</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：增强？ <sc xmlns =“http://www.wiley.com/namespaces/wiley”> l </ sc> l -phenylanine生产在<i xmlns =“http://www.wiley.com/namespaces/wiley”> Escherichia Coli </ i>通过<i XMLNS =“http://www.wiley.com/namespaces/wiley”> Vitreoscilla </ i> Vitreoscilla血红蛋白的异源表达</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-258995_thesis/0705012469921.html">PAT - PROCESS ANALYTICAL TECHNOLOGY IN CULTIVATION PROCESSES WITH RECOMBINANT ESCHERICHIA COLI</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=C. Kaiser&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">C. Kaiser,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=T. Peuker&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">T. Peuker,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=T. Bauch&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">T. Bauch,</a> <a href="/conference-foreign-258995/" target="_blank" rel="nofollow" class="tuijian_authcolor">IFAC Symposium on Computer Applications in Biotechnology .</a> <span>2007</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：重组大肠杆菌栽培过程中的PAT - 工艺分析技术</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061667685.html">Design of Escherichia coli host strains for improved recombinant protein purification: An approach that bridges the upstream and downstream realms of bioprocessing</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bartlow, Patrick Robert&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bartlow, Patrick Robert </a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：大肠埃希氏菌宿主菌株设计，用于改进重组蛋白的纯化：桥接生物加工上游和下游领域的方法</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040001773413.html">Asymmetric Catalysis In Organic Synthesis. Ryoji Nagoya (Nagoya University Japan). Wiley: New York (http://www.wiley.com/). 1994. Pages: 400 Pages Hardcover. Price: $94.95. ISBN: 0-471-57267-5</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <span>2001</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：有机合成中的不对称催化。名古屋良治（日本名古屋大学）。 Wiley：纽约（http://www.wiley.com/）。 1994.页数：400页精装。价格：$ 94.95书号：0-471-57267-5</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000127481561.html">Broadband homonuclear correlation spectroscopy driven by combined <mml:math altimg="si1.gif" overflow="scroll" xmlns:xocs="http://www.elsevier.com/xml/xocs/dtd" xmlns:xs="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://www.elsevier.com/xml/ja/dtd" xmlns:ja="http://www.elsevier.com/xml/ja/dtd" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:tb="http://www.elsevier.com/xml/common/table/dtd" xmlns:sb="http://www.elsevier.com/xml/common/struct-bib/dtd" xmlns:ce="http://www.elsevier.com/xml/common/dtd" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:cals="http://www.elsevier.com/xml/common/cals/dtd" xmlns:sa="http://www.elsevier.com/xml/common/struct-aff/dtd"><mml:mrow><mml:mtext>R</mml:mtext><mml:msubsup><mml:mrow><mml:mn>2</mml:mn></mml:mrow><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mi>v</mml:mi></mml:mrow></mml:msubsup></mml:mrow></mml:math> sequences under fast magic angle spinning for NMR structural analysis of organic and biological solids</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guangjin Hou&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guangjin Hou,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Si Yan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Si Yan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Julien Trébosc&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Julien Trébosc,</a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：由组合<MML：MATH ALTIMG =“SI1.GIF”overflow =“滚动”XMLNS：XOCS =“http://www.elsevier.com/xml/xocs/dtd”xmlns：xs =“http ：//www.w3.org/2001/xmlschema“xmlns：xsi =”http://www.w3.org/2001/xmlschema-instance“xmlns =”http://www.elsevier.com/xml/ja / dtd“xmlns：ja =”http://www.elsevier.com/xml/ja/dtd“xmlns：mml =”http://www.w3.org/1998/math/mathml“xmlns：tb =” http://www.elsevier.com/xml/common/table/dtd“xmlns：sb =”http://www.elsevier.com/xml/common/struct-bib/dtd“xmlns：ce =”http： //www.elsevier.com/xml/common/dtd“xmlns：xlink =”http://www.w3.org/1999/xlink“xmlns：cals =”http://www.elsevier.com/xml/ Common / Cals / DTD“XMLNS：SA =”http://www.elsevier.com/xml/common/struction-aff/dtd“> <mml：mrow> <mml：mtext> r </ mml：mtext> < MML：MSUBSUP> <MML：MROW> <MML：MN> 2 </ MML：MN> </ MML：MROW> <MML：MROW> <MML：MI> N </ MML：MI> </ MML：MROW> <MML：MROW> <MML：MI> V </ MML：MI> </ MML：MROW> </ MML：MSUBSUP> </ MML：MROW> </ MML：MATH> NMR结构的快速魔法角度旋转下的序列org分析ANIC和生物固体</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_oceanologia-limnologia-sinica_thesis/0201253921685.html">乳糖诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichia coli)中的表达</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=唐志红&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 唐志红</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=葛保胜&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,葛保胜</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=林凡&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,林凡</a> <span> <a href="/journal-cn-4981/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 海洋与湖沼 </a> </span> <span> . 2006</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_oceanologia-limnologia-sinica_thesis/0201253921858.html">中国对虾素在大肠杆菌(Escherichia coli)中的重组表达</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=薛剑峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 薛剑峰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=康翠洁&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,康翠洁</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王金星&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王金星</a> <span> <a href="/journal-cn-4981/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 海洋与湖沼 </a> </span> <span> . 2005</span><span>,第003期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130455374872.html">DNA FRAGMENT HC 250 PREPARED BY CHEMICAL SYNTHESIS, RECOMBINANT POLYPEPTIDE HC 250 OF MOLECULAR MASS 120-130 KDA SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS3 OF HEPATITIS C VIRUS PREPARED BY CULTIVATION OF THE STRAIN OF BACTERIUM ESCHERICHIA COLI AND STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RECOMBINANT POLYPEPTIDE HC 250 SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS3 OF HEPATITIS C VIRUS</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2073719C1 </span> <span> . 1997-02-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：化学合成制备的DNA片段HC 250，分子质量120-130 KDA的重组多肽HC 250具有显示能力，可将细菌培养的细菌的细菌和细菌的细菌培养分离，从而对产肝炎病毒C的蛋白质NS3进行抗原结合。 COLI-重组多肽HC 250的生产商，具有对肝炎病毒C基因编码蛋白NS3进行绑定抗体的能力。 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130455374873.html">DNA FRAGMENT HC 280 PREPARED BY CHEMICAL SYNTHESIS, RECOMBINANT POLYPEPTIDE HC 280 AT MOLECULAR MASS 120-130 KDA SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS4 OF HEPATITIS C VIRUS PREPARED AT CULTIVATION OF THE STRAIN OF BACTERIUM ESCHERICHIA COLI - STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RECOMBINANT POLYPEPTIDE HC 280 SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS4 OF HEPATITIS C VIRUS</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2073718C1 </span> <span> . 1997-02-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：化学合成制备的DNA片段HC 280，分子质量为120-130 KDA的重组多肽HC 280，具有结合抗原的能力，可将编码大肠杆菌的大肠杆菌NS4病毒的基因编码到大肠杆菌中。 COLI-重组多肽HC 280的生产商，具有对肝炎病毒C基因编码蛋白NS4进行抗原结合的能力 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130455358981.html">RECOMBINANT PLASMID DNA PURVGF DETERMINING SYNTHESIS OF SMALLPOX VIRUS STRAIN L-IVP GROWTH FACTOR TOGETHER WITH ESCHERICHIA COLI BETA-GALACTOSIDASE AND STRAIN OF BACTERIUM ESCHERICHIA COLI CONTAINING RECOMBINANT PLASMID DNA PURVGF - A PRODUCER OF SMALLPOX VIRUS STRAIN L-IVP GROWTH FACTOR</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2089612C1 </span> <span> . 1997-09-10</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：重组质粒DNA PURVGF与大肠杆菌β-半乳糖酶和含重组质粒DNA PURVGF的大肠杆菌细菌一起合成了痘病毒L-IVP生长因子-小ROW病毒的生产者 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704021881123','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche"> <!-- <a href="javascript:void(0);" onclick="link_analysis('/shoppingcart/auth/list.html',this)">购物车</a>--> </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704021881123down" data-source="7," data-out-id="mnY9xRO0VnIoy9T6ZR8Udw==," data-f-source-id="7" data-title="Analysis of the efficiency of recombinant <i xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia?coliEscherichia?coli strain cultivation in a gas‐vortex bioreactor" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="15016" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Analysis of the efficiency of recombinant <i xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia?coliEscherichia?coli strain cultivation in a gas‐vortex bioreactor"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>