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首页> 外文期刊>Biotechnology and Applied Biochemistry >Enhanced production of podophyllotoxin, kaempferol, and quercetin from callus culture of Dysosma pleianthaDysosma pleiantha (Hance) Woodson: An endangered medicinal plant
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Enhanced production of podophyllotoxin, kaempferol, and quercetin from callus culture of Dysosma pleianthaDysosma pleiantha (Hance) Woodson: An endangered medicinal plant

机译：从愈伤组织培养的增强的鼠毒素毒素，kaempferol和槲皮素的生产 Dysosma pleiantha Dysosma pleiantha （Hance）Woodson：濒临灭绝的药用植物

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Abstract > Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and enhanced production of pharmaceutically important podophyllotoxin, kaempferol, and quercetin from callus culture of D. pleiantha under the influence of different additives. Best callus induction was achieved in Gamborg's medium (B5) with 1 mg/L 2,4‐dichlorophenoxyacetic acid (2,4‐D) along with 0.2 mg/L kinetin under dark condition. Tender leaves of D. pleiantha showed the maximum of 86% callus induction among the different explants tested. Highest leaf callus proliferation was noted in B5 medium with 1 mg/L 2,4‐D incubated under complete darkness. In addition, it was found that B5 medium with 1 mg/L 2,4‐D along with 2 g/L peptone produced more leaf callus biomass and enhanced production of podophyllotoxin (16.3‐fold), kaempferol (12.39‐fold), and quercetin (5.03‐fold) compared to control. Therefore, D. pleiantha callogenesis can provide an alternative source for enhanced production of secondary compounds regardless of the exploitation of its natural plant population. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > Dysosma pleiantha </ i> （Hance）伍德森是濒危中药草药之一，由台湾山地部落的药用特性高度重视。本研究旨在通过优化合适的培养基，碳源培养条件，增强药学上重要的波脱毒素，Kaempferol和来自愈伤组织蛋白的槲皮素来开发一种有效的愈伤组织生物量方案 d。 pleiantha </ i> 在不同添加剂的影响下。在Gamborg的培养基（B5）中获得最佳愈伤组织诱导，其中1mg / L 2,4-二氯苯氧基乙酸（2,4-D）以及0.2mg / L inteNin的暗条件下。嫩叶 d。 pleiantha </ i> 在测试的不同外植体中显示最多86％的愈伤组织诱导。在B5培养基中注意到最高叶片愈伤组织增殖，1mg / L 2,4-D在完全黑暗下培养。此外，发现B5培养基含有1mg / L 2,4-d，还与2g / l蛋白胨产生更多的叶子愈伤组织生物质，并增强鼠毒素毒素（16.3倍），Kaempferol（12.39倍）的生产与对照相比槲皮素（5.03倍）。所以， d。 pleiantha </ i> 无论自然植物种群的利用如何，胼uEncy都可以提供增强的二次化合物的替代来源。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2020年第1期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bajiaolian&option=203" rel="nofollow">bajiaolian;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=callogenesis&option=203" rel="nofollow">callogenesis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dysosma pleiantha&option=203" rel="nofollow">Dysosma pleiantha;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=kaempferol&option=203" rel="nofollow">kaempferol;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=quercetin&option=203" rel="nofollow">quercetin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=podophyllotoxin&option=203" rel="nofollow">podophyllotoxin;</a> </p> <div class="translation"> 机译：八角莲;call o Genesis;D又SOS map类ant哈;卡EMP反而OL;去二测体能;pod op还有LL o toxin; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881094.html">Enhanced production of podophyllotoxin, kaempferol, and quercetin from callus culture of <i >Dysosma pleianthaDysosma pleiantha (Hance) Woodson: An endangered medicinal plant</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2020</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从愈伤组织培养的增强的鼠毒素毒素，kaempferol和槲皮素的生产 Dysosma pleiantha </ i> Dysosma pleiantha （Hance）Woodson：濒临灭绝的药用植物</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070402970113.html">An efficient regeneration system via direct and indirect organogenesis for the medicinal plant Dysosma versipellis (Hance) M. 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