Development of a microchannel emulsification process for pancreatic beta cell encapsulation

Bitar Christina M.E.; Markwick Karen E.; Tre?ová Du?ana; Kroneková Zuzana; Pelach Michal; Selerier Chloé M.O.; Dietrich James; Lacík Igor; Hoesli Corinne A.

摘要

Abstract > In this study, we developed a high‐throughput microchannel emulsification process to encapsulate pancreatic beta cells in monodisperse alginate beads. The process builds on a stirred emulsification and internal gelation method previously adapted to pancreatic cell encapsulation. Alginate bead production was achieved by flowing a 0.5–2.5% alginate solution with cells and CaCO <sub>3</sub> across a 1‐mm thick polytetrafluoroethylene plate with 700?×?200?μm rectangular straight‐through channels. Alginate beads ranging from 1.5–3?mm in diameter were obtained at production rates exceeding 140?mL/hr per microchannel. Compared to the stirred emulsification process, the microchannel emulsification beads had a narrower size distribution and demonstrated enhanced compressive burst strength. Both microchannel and stirred emulsification beads exhibited homogeneous profiles of 0.7% alginate concentration using an initial alginate solution concentration of 1.5%. Encapsulated beta cell viability of 89?±?2% based on live/dead staining was achieved by minimizing the bead residence time in the acidified organic phase fluid. Microchannel emulsification is a promising method for clinical‐scale pancreatic beta cell encapsulation as well as other applications in the pharmaceutical, food, and cosmetic industries. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 在这项研究中，我们开发了一种高通量微通道乳化过程，用于在单分散的藻酸盐珠粒中封装胰腺β细胞。该方法在预先适用于胰腺细胞包封的搅拌乳化和内凝胶化方法上。通过用细胞和可可流动0.5-2.5％的藻酸盐溶液来实现海藻酸盐珠生产 <sub> 3 </ sub> 穿过1毫米厚的聚四氟乙烯板，具有700Ω×200？μm矩形直通通道。在每微型通道超过140μl/小时的生产速率下获得直径为1.5-3Ωmm的藻酸盐珠。与搅拌的乳化过程相比，微通道乳化珠具有较窄的尺寸分布，并证明了增强的压缩爆发强度。微通道和搅拌乳化珠均具有0.7％藻酸盐浓度的均匀曲线，初始藻酸盐溶液浓度为1.5％。通过最小化酸化有机相流体中的珠子停留时间来实现基于实时/死染料的89〜±2％的封装β细胞活力。微通道乳化是临床规模胰腺β细胞包封以及制药，食品和化妆品行业的其他应用方法。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bitar Christina M.E.&option=202" target="_blank" rel="nofollow">Bitar Christina M.E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Markwick Karen E.&option=202" target="_blank" rel="nofollow">Markwick Karen E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tre?ová Du?ana&option=202" target="_blank" rel="nofollow">Tre?ová Du?ana;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kroneková Zuzana&option=202" target="_blank" rel="nofollow">Kroneková Zuzana;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pelach Michal&option=202" target="_blank" rel="nofollow">Pelach Michal;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Selerier Chloé M.O.&option=202" target="_blank" rel="nofollow">Selerier Chloé M.O.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dietrich James&option=202" target="_blank" rel="nofollow">Dietrich James;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lacík Igor&option=202" target="_blank" rel="nofollow">Lacík Igor;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hoesli Corinne A.&option=202" target="_blank" rel="nofollow">Hoesli Corinne A.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical EngineeringMcGill UniversityMontreal Quebec Canada;</p> <p>Department of Chemical EngineeringMcGill UniversityMontreal Quebec Canada;</p> <p>Department for Biomaterials ResearchPolymer Institute of the Slovak Academy of SciencesBratislava Slovakia;</p> <p>Department for Biomaterials ResearchPolymer Institute of the Slovak Academy of SciencesBratislava Slovakia;</p> <p>Department for Biomaterials ResearchPolymer Institute of the Slovak Academy of SciencesBratislava Slovakia;</p> <p>Department of Chemical EngineeringMcGill UniversityMontreal Quebec Canada;</p> <p>Advanced Radio Frequency Systems Laboratory CMC MicrosystemsUniversity of ManitobaWinnipeg Manitoba Canada;</p> <p>Department for Biomaterials ResearchPolymer Institute of the Slovak Academy of SciencesBratislava Slovakia;</p> <p>Department of Chemical EngineeringMcGill UniversityMontreal Quebec Canada;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=alginate&option=203" rel="nofollow">alginate;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cellular therapy&option=203" rel="nofollow">cellular therapy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=emulsion&option=203" rel="nofollow">emulsion;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=encapsulation&option=203" rel="nofollow">encapsulation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microchannel&option=203" rel="nofollow">microchannel;</a> </p> <div class="translation"> 机译：藻酸盐;细胞疗法;乳液;封装;微通道; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021880103.html">Development of a microchannel emulsification process for pancreatic beta cell encapsulation</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bitar Christina M.E.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bitar Christina M.E.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Markwick Karen E.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Markwick Karen E.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tre?ová Du?ana&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tre?ová Du?ana,</a> <a href="/journal-foreign-15005/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology Progress .</a> <span>2019</span><span>,第6期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于胰腺β细胞包封的微通道乳化过程的研制</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/07040791144.html">A new approach for pancreatic tissue engineering: human endometrial stem cells encapsulated in fibrin gel can differentiate to pancreatic islet beta-cell</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Azadeh Niknamasl&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Azadeh Niknamasl,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seyed Nasser Ostad&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Seyed Nasser Ostad,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mansoureh Soleimani&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mansoureh Soleimani,</a> <a href="/journal-foreign-15882/" target="_blank" rel="nofollow" class="tuijian_authcolor">Cell biology international. .</a> <span>2014</span><span>,第10期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：胰腺组织工程的新方法：用纤维蛋白凝胶包封的人类子宫内膜干细胞可以分化为胰岛β细胞</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022028207.html">A new approach for pancreatic tissue engineering: human endometrial stem cells encapsulated in fibrin gel can differentiate to pancreatic islet beta-cell</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Azadeh Niknamasl&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Azadeh Niknamasl,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seyed Nasser Ostad&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Seyed Nasser Ostad,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mansoureh Soleimani&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mansoureh Soleimani,</a> <a href="/journal-foreign-15882/" target="_blank" rel="nofollow" class="tuijian_authcolor">Cell biology international. .</a> <span>2014</span><span>,第10期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：胰组织工程的一种新方法：包封在纤维蛋白凝胶中的人子宫内膜干细胞可以分化为胰岛β细胞</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_scale-up-manufacturing-cell-based-therapies-v_thesis/0205173472.html">DEVELOPMENT OF MICROCHANNEL EMULSIFICATION AS A NOVEL CELL ENCAPSULATION TECHNOLOGY</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Karen Markwick&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Karen Markwick,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Corinne Hoesli&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Corinne Hoesli </a> <a href="/conference-foreign-2559/" target="_blank" rel="nofollow" class="tuijian_authcolor">Scale-up and manufacturing of cell-based therapies V .</a> <span>2017</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：微通道乳化作为新型细胞包裹技术的发展</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061250446.html">Cryopreservation effects on a pancreatic substitute comprised of beta cells or recombinant myoblasts encapsulated in nonadhesive and adhesive alginate hydrogels.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ahmad, Hajira Fatima.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ahmad, Hajira Fatima. </a> <span>2012</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：冷冻保存对胰岛替代物的影响，胰岛替代物由非粘附性和粘附性藻酸盐水凝胶封装的β细胞或重组成肌细胞组成。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040003478991.html">Biosynthesis of betagranin in pancreatic beta-cells. Identification of a chromogranin A-like precursor and its parallel processing with proinsulin.</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=J C Hutton&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">J C Hutton,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=H W Davidson&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">H W Davidson,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=K A Grimaldi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">K A Grimaldi,</a> <span>1987</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：贝塔格拉宁在胰腺β细胞中的生物合成。嗜铬粒蛋白A样前体的鉴定及其与胰岛素原的平行加工。</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100044307492.html ">Co-encapsulation of bioengineered IGF-II-producing cells and pancreatic islets: effect on beta-cell survival.</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jourdan, Guillaume&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jourdan, Guillaume,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dusseault, Julie&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Dusseault, Julie,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Benhamou, Pierre-Yves&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Benhamou, Pierre-Yves,</a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：生物工程化的产生IGF-II的细胞和胰岛的共包封：对β细胞存活的影响。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-chongqing-technology-business-university-natural-science-edition_thesis/0201248187330.html">用于高功率半导体激光器列阵散热的微通道热沉的研制</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=唐裕霞&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 唐裕霞</a> <span> <a href="/journal-cn-4532/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 重庆工商大学学报（自然科学版） </a> </span> <span> . 2009</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_experimental-technology-management_thesis/020125419065.html">微通道过程强化实验装置研制与实践</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王瑶&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王瑶</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王刚&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王刚</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=匡国柱&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,匡国柱</a> <span> <a href="/journal-cn-2730/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 实验技术与管理 </a> </span> <span> . 2015</span><span>,第008期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-university-jinan-science-technology_thesis/0201250587151.html">激光加工的石英微通道用于细胞操纵的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨子义&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨子义</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=秦水介&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,秦水介</a> <span> <a href="/journal-cn-4703/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 济南大学学报（自然科学版） </a> </span> <span> . 2006</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chemical-journal-chinese-universities_thesis/0201231591576.html">新型微盘传感器的研制及用于单细胞释放NO监测</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杜富滢&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杜富滢</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄卫华&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,黄卫华</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=吴文展&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,吴文展</a> <span> <a href="/journal-cn-10958/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 高等学校化学学报 </a> </span> <span> . 2007</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230630941.html">用于细胞破裂的微流控生物芯片的研制</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈兴&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈兴</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=崔大付&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,崔大付</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘长春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘长春</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2006</span><span>,第011期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-6060_thesis/020221590340.html">用于卵母细胞保护剂添加过程的微流体通道的浓度场模拟</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Yun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Yang Yun</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨云&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨云</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou Xinli&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Zhou Xinli</a> <span> <a href="/conference-cn-6060/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第八届全国低温生物医学及器械学术大会 </a> <span> <span> . 2013</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020311413992.html">用于检测细胞电阻的PDMS微流控芯片的研制</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=许蓉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 许蓉</a> <span> . 2009</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120214973564.html">一种用于液体乳化过程的激光内雕刻微通道反应器</a> <b>[P]</b> . <span> 中国专利： CN215389194U </span> <span> . 2022-01-04</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120101322454.html">一种用于细胞贴壁生长过程中温度监测的无线多通道系统</a> <b>[P]</b> . <span> 中国专利： CN110438002A </span> <span> . 2019-11-12</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130446738114.html">The preparation of encapsulated pancreatic beta islet cells treated with nicotinamide from piglets able to produce porcine insulin</a> <b>[P]</b> . <span> 外国专利：  NZ502473A </span> <span> . 2002-09-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从能产生猪胰岛素的仔猪中制备烟酰胺处理的胰岛胰岛胰岛细胞 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130421814369.html">METHOD FOR PRODUCING RABBIT PANCREATIC BETA CELLS AND COMPOSITION FOR STIMULATING NATURAL INSULIN DEVELOPMENT IN PATIENT</a> <b>[P]</b> . <span> 外国专利：  RU2450052C2 </span> <span> . 2012-05-10</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：制备兔胰腺β细胞的方法和用于刺激患者天然胰岛素发育的组合物 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130476122922.html">Process for the production of an insulin-producing cell line of pancreatic beta cells</a> <b>[P]</b> . <span> 外国专利：  US4332893A </span> <span> . 1982-06-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：胰β细胞的胰岛素产生细胞系的生产方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704021880103','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704021880103down" data-source="7," data-out-id="DH9j2jxsWKv+FlaUvzGkGfp/ovhurVAPNziFomboM2n0tQyoRDVFbQtyEVWQ8S8d," data-f-source-id="7" data-title="Development of a microchannel emulsification process for pancreatic beta cell encapsulation" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="0704021880103" id="docid"> <input type="hidden" value="15005" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Development of a microchannel emulsification process for pancreatic beta cell encapsulation"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/page.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/reference.js?v=5.8.0"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.8.0"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>