Amniotic fluid lactic acid and matrix metalloproteinase‐8 levels at the time of fetal surgery for a spine defect: association with subsequent preterm prelabour rupture of membranes

摘要

Objective In utero fetal surgery to correct incomplete closure of the spinal cord lessens the extent of permanent damage but is associated with preterm prelabour rupture of membranes ( PPROM ). We determined whether compounds in amniotic fluid collected at the time of surgery predicted subsequent development of PPROM . Design Prospective study. Setting Hospitals in Sao Paulo, Brazil. Population Twenty‐four consecutive pregnant women at 24–26 weeks of gestation seen between February and October 2017 with a singleton pregnancy underwent in utero surgery to correct an open spinal defect in their fetus. Methods Amniotic fluid was tested for lactic acid, matrix metalloproteinase 2 ( MMP ‐2), MMP ‐8, MMP ‐9 and interleukin‐6 ( IL ‐6) by enzyme‐linked immunosorbent assay. Clinical data were collected after completion of all laboratory studies. Main outcome measure Amniotic fluid concentration of compounds in women with or without PPROM . Results Preterm prelabour rupture of membranes occurred in seven (29.2%) women. There were no differences in maternal age, gravidity, parity, race, history of caesarean sections or fetal gender between women with or without PPROM . Length of surgery, days of wound healing and length of hospital stay were also indistinguishable. The median concentrations of MMP ‐8 (1.7 versus 0.6 ng/ml; P = 0.0041) and lactic acid (7.1 versus 5.9 m m ; P = 0.0181) were higher in women with PPROM . The amniotic fluid MMP ‐8 level was also negatively correlated with gestational age at delivery (Spearman r = ?0.4217, P = 0.0319). Conclusion Differences in susceptibility to develop PPROM are present before fetal surgery. An increase in anaerobic glycolysis, evidenced by the intra‐amniotic lactic acid level, may enhance MMP ‐8 production and weaken maternal and fetal membranes. Tweetable abstract Matrix metalloproteinase‐8 and lactic acid in amniotic fluid predict preterm prelabour rupture of membranes.