Marine-derived natural polymer-based bioprinting ink forbiocompatible, durable, and controllable 3D constructs

摘要

3D bioprinting (3DBP)is a rapid solid-form fabrication method with a high degree of automation andreproducibility for constructing structural bioscaffolds. However, the development of the 3DBP fieldhas been slowed due to difficulty in acquiring suitable ink materials especially with natural polymersthat satisfy all requirements, such as printability, mechanical integrity, and biocompatibility. In thisstudy, a new 3DBP ink of bioengineered sea anemone-derived silk-like protein (aneroin)was usedbased on its durable mechanical properties and biodegradability in previous studies. The hyaluronicacid and mussel adhesive protein (MAP)were applied for improved printability and cell adhesiveness,respectively. The aneroin-based 3DBP ink (named aneroin ink)was solidified in a few second bydityrosine photo-crosslinking, and its fast reaction was suitable for noncollapsed spaces in printed 3Dconstructs. Actual-sized human ear, vascular graft, and rectangular multi-layered lattice werebioprinted with high controllability and durable structural integrity. Thus, the developed aneroin inkshowed good printability, structural integrity, and biocompatibility for successful application to theconstruction of various 3D shaped bioscaffolds in tissue and biomedical engineering fields.