Controlled synthesis of an enzyme-inorganic crystal composite assembled into a 3D structure with ultrahigh enzymatic activity

摘要

In this study, we report a method for the controlled synthesis of an enzyme-inorganic crystal composite assembled into a 3D structure with flower-like shape, which shows an ultrahigh enzymatic activity. Using ethylenediaminetetraacetic acid (EDTA) as the chelating compound, the addition of a protein aqueous solution in phosphate saline buffer into a water solution containing Cu(II) resulted in a 3D architecture made of protein and flower-like shaped copper phosphate crystals with controllable size ranging from approximately 1 mu m to 15 mu m. The incorporated laccase showed ultrahigh activity, which was about 7-11 times higher compared to free laccase in solution. The immobilized laccase-copper phosphate hybrid microflowers can be applied for rapid and sensitive detection of phenol in water. Repeated use of immobilized laccase microflowers was demonstrated for 60 cycles during a period of two months.