Modulating the DNA strand-displacement kinetics with the one-sided remote toehold design for differentiation of single-base mismatched DNA

摘要

A one-sided remote toehold design was proposed to provide the fine control over strand-displacement reaction kinetics with simplicity and versatility. The significantly improved single-base mismatch differentiating ability of reactions was achieved for strand-displacement reaction systems with the rate-saturated toehold length.