HISTOCHEMICAL STUDIES ON SPOROGENOUS TISSUE AND PERIPLASMODIAL TAPETUM IN THE ANTHER OF CREPIS JAPONICA (ASTERACEAE)

摘要

The present study deals with the qualitative localization of polysacoharides, and total proteins in the developing sporogenous tissue and periplasmodium tapetum of Crepis japonica. This study revealed that: (1) Microsporocyte possesses high concentration of proteins, but lacks storage starch; (2) Tapetum is metabolically a very active tissue; (3) Periplasmodial fluid lacks PAS-positive granules; (4) Cells of the endothecium, shortly after meiosis, exhibit intense accumulation of storage starch whichdisappear completely by the time mature pollen and endothecium are formed; (5) Callose gives PAS positive test; (6) Maturing pollen grains are gorged with polysaccharides and cytoplasmic proteins. The data obtained were evaluated in the light of previous histochemical works.