Polymorphism of genes encoding for ryanodine receptor, growth hormone, leptin and MYC protooncogene protein and meat production in Duroc pigs

摘要

This study investigated the associations of ryanodine receptor 1 (RYR1), growth hormone (GH1) leptin (LEP) and MYC protooncogene protein (MYC) with parameters of growth rate and fat deposition in 117 pigs of Duroc breed (sex ratio 1 : 1). PCR-RFLPs were genotyped for RYR1 (HhaI), GHI (MspI and HaeII), LEP (HinfI), and MYC (HpaII) loci. The following allele frequencies were found: GHI-HaeII + = 0.54 and - = 0.46; GH1-MspI + = 0.85 and - = 0.15; LEP T = 0.65 and C = 0.35 and MYC A = 0.82 and B = 0.18, respectively. RYR1 gene was monomorphic (C = 1.00). Neither of GH1 loci (HaeII and MspI) showed any effect on variability of meat performance traits under study apart from GH1-MspI, which affected average daily gain (+/+ = 582.44 +/- 7.02 g/d vs. +/- 599.85 +/- 8.54 g/d; P less than or equal to 0.05). LEP influenced average daily gain (TT = 578.55 +/- 10.73 g/d vs. TC = 597.39 +/- 11.27 g/d, P less than or equal to 0.01) and backfat thickness (TT = 9.04 +/- 0.52 mm vs. TC = 8.03 +/- 0.54 mm, P less than or equal to 0.01) and percentage of lean meat content (TT = 59.88 +/- 0.42% vs. TC = 60.36 +/- 0.54% and CC = 60.91 +/- 0.52%; P less than or equal to 0.05). MYC genotypes affected average daily gain (AB = 565.22 +/- 10.79 g/d vs. AA = 594.82 +/- 9.78 g/d, P less than or equal to 0.001; AB vs. BB = 608.66 +/- 19.28 g/d, P less than or equal to 0.05) and average daily gain in the test period (AA = 966.93 +/- 19.94 g/d vs. AB = 929.87 +/- 21.96 g/d, P less than or equal to 0.01). These results have to be confirmed on another population with larger number of animals of Duroc breed.