Electrochemical study of ferrocenemethanol-modified layered double hydroxides composite matrix: Application to glucose amperometric biosensor

摘要

A novel amperometric glucose sensor based on co-inimobilization of ferrocenemethanol (MeOHFc) and glucose oxidase (GOD) in the layered double hydroxides (LDHs) was described. MeOHFc immobilized in LDHs played effectively the role of an electron shuttle and allowed the detection of glucose at 0.25 V (versus SCE), with dramatically reduced interference from easily oxidizable constituents. The sensor (LDHs/MeOHFc/GOD) exhibited a relatively fast response (response time was about 5 s), low detection limit (3 mu M), and high sensitivity (ca. 60 mA M-1 cm(-2)) with a linear range of 6.7 x 10(-6) to 3.86 x 10(-4) M of glucose. Apparent Michaelis-Menten constant was calculated to be 2.25 mm. (c) 2007 Elsevier B.V. All rights reserved.