Combination of dynamic magnetophoretic separation and stationary magnetic trap for highly sensitive and selective detection of Salmonella typhimurium in complex matrix

摘要

Foodbome illnesses have always been a serious problem that threats public health, so it is necessary to develop a method that can detect the pathogens rapidly and sensitively. In this study, we designed a magnetic controlled microfluidic device which integrated the dynamic magnetophoretic separation and stationary magnetic trap together for sensitive and selective detection of Salmonella typhimurium (S. typhimurium). Coupled with immunomagnetic nanospheres (IMNs), this device could separate and enrich the target pathogens and realize the sensitive detection of target pathogens on chip. Based on the principle of sandwich immunoassays, the trapped target pathogens identified by streptavidin modified QDs (SA-Q,Ds) were detected under an inverted fluorescence microscopy. A linear range was exhibited at the concentration from 1.0 x 10(4) to 1.0 x 10(6) colony-forming units/mL (CFU/mL), the limit of detection (LOD) was as low as 5.4 x 10(3) CFU/mL in milk (considering the sample volume, the absolute detection limit corresponded to 540 CFU). Compared with the device with stationary magnetic trap alone, the integrated device enhanced anti-interference ability and increased detection sensitivity through dynamic magnetophoretic separation, and made the detection in complex samples more accurate. In addition, it had excellent specificity and good reproducibility. The developed system provides a rapid, sensitive and accurate approach to detect pathogens in practice samples. (C) 2015 Elsevier B.V. All rights reserved.