Serine/Threonine Protein Kinases from Bacteria, Archaea and Eukarya Share a Common Evolutionary Origin Deeply Rooted in the Tree of Life

摘要

Abstract The main family of serine/threonine/tyrosine protein kinases present in eukarya was defined and described by Hanks et al . in 1988 ( Science , 241 , 42–52). It was initially believed that these kinases do not exist in bacteria, but extensive genome sequencing revealed their existence in many bacteria. For historical reasons, the term “eukaryotic-type kinases” propagated in the literature to describe bacterial members of this protein family. Here, we argue that this term should be abandoned as a misnomer, and we provide several lines of evidence to support this claim. Our comprehensive phylostratigraphic analysis suggests that Hanks-type kinases present in eukarya, bacteria and archaea all share a common evolutionary origin in the lineage leading to the last universal common ancestor (LUCA). We found no evidence to suggest substantial horizontal transfer of genes encoding Hanks-type kinases from eukarya to bacteria. Moreover, our systematic structural comparison suggests that bacterial Hanks-type kinases resemble their eukaryal counterparts very closely, while their structures appear to be dissimilar from other kinase families of bacterial origin. This indicates that a convergent evolution scenario, by which bacterial kinases could have evolved a kinase domain similar to that of eukaryal Hanks-type kinases, is not very likely. Overall, our results strongly support a monophyletic origin of all Hanks-type kinases, and we therefore propose that this term should be adopted as a universal name for this protein family. Graphical abstract Display Omitted Highlights ? We argue that the term “eukaryotic-type protein kinases” is a misnomer. ? These kinases are present and abundant in bacteria, archaea and eukarya. ? Our phylostratigraphy analysis places the origin of this protein family in LUCA. ? Convergent evolution and massive horizontal transfer scenarios are ruled out. ? We propose to replace the term “eukaryotic-type” by “Hanks-type” protein kinases.