Synthesis and Characterization of Calcium Phosphate Ceramic/(Poly(vinyl alcohol)-Polycaprolactone) Bilayer Nanocomposites-A Bone Tissue Regeneration Scaffold

摘要

beta Tricalcium phosphate ceramic was used to reinforce nanofibers in composite mats produced via electrospinning of poly(vinyl alcohol) (PVA), polycaprolactone (PCL) and (PVA: PCL) bilayers. The role of TCP ceramic on morphology of nanocomposites, crystalline structure, functional groups and thermal behaviour of nanocomposites were characterized by SEM, EDAX, XRD, FTIR and DSC analysis. Ultrathin cross-sections of the obtained nanocomposites were morphologically investigated with SEM and all fabricated composites consisted of fibers with average fiber diameter (AFD) around 100 nm except PCL-TCP fibers having AFD in the range of 608 nm. XRD profile presented the main peaks of beta-TCP (JCPDS 090169 and JCPDS 70-2065). The characteristic absorption bands of TCP were also identified by FTIR in all the composites. The thermal stability was enhanced after adding TCP filler particles in all the polymer composites. The porosity of PCL-TCP was found around 63% and (PVA-PCL: TCP) composite was found to be 58%. The biocompatibility of the (PVA-PCL: TCP) composite scaffold has also been investigated by culturing MG-63 osteoblast cells on it; primary results showed that the cells adhered and proliferated well on the composite scaffold.