Capillary electrophoresis with stationary nanogel zones of galactosidase and Erythrina cristagalli lectin for the determination of β(1–3)-linked galactose in glycans

摘要

Highlights?Fixed zones of enzyme or lectin were created in the capillary with nanogel.?β(1–4)-galactosidase andErythrina cristagallilectin were used for hydrolysis and capture.?β(1–4)-galactosidase maintained activity for much longer in nanogel than in aqueous buffer.?β(1–3)-galactose linkages in glycans were identified.?Factors that enhance the enzyme performance and the separation are described.AbstractA thermally responsive nanogel is used to create stationary zones of enzyme and lectin in a separation capillary. Once patterned in the capillary, analyte is driven through the zone, where it is converted to a specific product if an enzyme is used or captured if a lectin is used. These stationary zones are easily expelled after the analysis and then re-patterned in t