Pore size effect of mesoporous silica stationary phase on the separation performance of microfabricated gas chromatography columns

摘要

Microfabricated semi-packed gas chromatography (GC) columns coated with two kinds of mesoporous silica (MS) stationary phase have been explored and compared in this paper. The micro GC (mu GC) columns are fabricated by microelectromechanical system (MEMS) technique. MSs with pore size of 2 and 5 nm are prepared using two different soft-templates and deposited on the inner surface of the mu GC columns by dip-coating. For the first time, the influence of pore size of MS stationary phase on the separation performance in a mu GC column has been investigated. As demonstrated, the mixtures of light alkanes (C1-C4) or heavy alkanes (C5-C10) can be well separated in both columns, where the separation resolution is always higher than 1.25 except for C1-C2 and C5-C6. Considering the pore size effect on the separation performance, it is shown that analytes, especially those with bigger size, display longer retention time in the column with larger pore size. For C10, the retention time in 5 nm-MS column is 200% longer than that in 2 nm-MS one. (C) 2018 Elsevier B.V. All rights reserved.