Interest of achiral-achiral tandem columns for impurity profiling of synthetic drugs with supercritical fluid chromatography

摘要

Highlights?Coupling two columns is an easy way to assemble selectivities in SFC.?Tandem columns improve peak capacity and sensitivity over single columns.?Some column combinations are more effective than others.?The analysis time reduction with tandem columns is currently restricted by system pressure limits.AbstractTo achieve the most complete impurity profiling of synthetic drugs with a single chromatographic technique, high resolution is required, which may be gained with a combination of high efficiency and versatile selectivity, allowing to separate most similar analytes. Compared to a single-column chromatographic method, coupling complementary stationary phases promises both an increase in efficiency and an increase in selectivity possibilities. With supercritical fluid chromatography (SFC), the use of long columns is facilitated by the low viscosity of the mobile phase. In this paper, we inve