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Nanoparticle Synthesis, Characterization, and Ecotoxicity: A Research-Based Set of Laboratory Experiments for a General Chemistry Course

机译:纳米粒子合成,表征和生态毒性:一套基于研究的一般化学课程实验室实验

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摘要

A series of laboratory experiments were developed to introduce first-year chemistry students to nanoscience through a green chemistry approach. Students made and characterized the stability of silver nanoparticles using two different methods: UV-visible spectroscopy and dynamic light scattering. They then assessed the ecotoxicity of silver nano particles in Escherichia coli sublethal growth curve assays. Bacterial growth was monitored via optical density and carbon dioxide measurements. Finally, students designed and implemented their own nanomaterial characterization and ecotoxicity experiments based upon the insights gained in previous tests. The experiments translated current research in sustainable nanomaterials to an undergraduate population while introducing them to research methodology and experiences early in their undergraduate career. Moreover, the experiments emphasized the interdisciplinary nature of the sciences to provide real-world applications and connect concepts learned within general chemistry lecture and lab to other classes commonly taken by first-year STEM students, including biology, materials science, and environmental sciences.
机译:开发了一系列实验室实验,通过绿色化学方法将一年化学学生引入纳米科学。使用两种不同的方法制作并表征银纳米粒子的稳定性:UV可见光谱和动态光散射。然后,它们评估了大肠杆菌核心生长曲线测定中银纳米颗粒的生态毒性。通过光密度和二氧化碳测量监测细菌生长。最后,基于先前测试中获得的见解,学生设计和实施了自己的纳米材料表征和生态毒性实验。该实验将可持续纳米材料的当前研究转化为本科人口,同时将它们引入本科生生涯的早期研究方法和经验。此外,实验强调了科学的跨学科性质,以提供现实世界的应用,并在一般化学讲座和实验室中学到的概念,以及一年内常常采取的其他课程,包括生物学,材料科学和环境科学。

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