Co-electrospun cellulose diacetate-graft-poly(ethylene terephthalate) and collagen composite nanofibrous mats for cells culture

摘要

In this study, a kind of novel composite nanofibrous mats with cellulose diacetate-graft-poly(ethylene terephthalate) (CDA-g-PET) and Type I collagen were obtained by electrospinning and five different ratios of CDA-g-PET/Type I collagen (1:0, 1:0.1, 1:0.2, 1:0.3, 1:0.4) were set to explore the effects of components on its properties. The scanning electron microscope images demonstrated that the formation of uniform and smooth nanofibers with free beads. Compared with pure CDA-g-PET mats, the composite materials transformed into hydrophilic ones with increasing the content of Type I collagen. Moreover, the tensile strength of the composite nanofibrous mats got higher, while its water vapor transmission rate got lower after blending with Type I collagen. In addition, the cell proliferation and adhesion on composite nanofibrous mats were improved according by the results of bone marrow mesenchymal stem cells (BMSCs) culturing experiments. Especially, the cells growth attained optimum when the CDA-g-PET/Type I collagen ratio reached 1:0.3 and 1:0.4, where showed no significant difference. In consequence, the above results indicated that the novel composite nanofibrous mats consisted of CDA-g-PET and Type I collagen obtained by electrospinning combined good mechanical strength of CDA-g-PET and excellent biological functions of Type I collagen. The nanoscale structure might mimic the nanofibrous extracellular matrix feature, which present the potential use in cells culture.