Destruction complex dynamics: Wnt/beta-catenin signaling alters Axin-GSK3 beta interactions in vivo

摘要

The central regulator of the Wnt/beta-catenin pathway is the Axin/APC/GSK3 beta destruction complex (DC), which, under unstimulated conditions, targets cytoplasmic beta-catenin for degradation. How Wnt activation inhibits the DC to permit beta-catenin-dependent signaling remains controversial, in part because the DC and its regulation have never been observed in vivo. Using bimolecular fluorescence complementation (BiFC) methods, we have now analyzed the activity of the DC under near-physiological conditions in Drosophila. By focusing on well-established patterns of Wnt/Wg signaling in the developing Drosophila wing, we have defined the sequence of events by which activated Wnt receptors induce a conformational change within the DC, resulting in modified Axin-GSK3 beta interactions that prevent beta-catenin degradation. Surprisingly, the nucleus is surrounded by active DCs, which principally control the degradation of beta-catenin and thereby nuclear access. These DCs are inactivated and removed upon Wnt signal transduction. These results suggest a novel mechanistic model for dynamic Wnt signal transduction in vivo.