In vitro growth studies of rice, maize and ground nut seeds using culture cotton method

摘要

Methylobacterium, bioinoculant can be used to improve plant growth. In vitro Groth studies of various seeds were observed using culture cotton method which is not used previously. Methylotrophs a potent bioinoculant to improve crop production. These organism oxidize environmental methane as a sole source of carbon (Quayle, 1961) for their growth either aerobically (Hallem etal, 2004) or anaerobic environment. Green and Bousefield stated that these organisms are predominantly present in paddy field soil and phyllosphere because of high methane emission in paddy field. (New Scientist magazine, 1989). The methane source in the paddy field supports the growth of methylotrophs (Charles etal, 2005). Methylotrophs can be easily cultured in laboratory usingsome specialized media. Methylotrophs are agriculturally important organisms by producing growth hormone vitamins and pigments (Corpe, 1985; Mahadevan, 1985; Holland and Polacco, 1994; Rohmer, 1999; Koeing, 2002). Various invitro -ulturing of plants have been introduced so for to identify Agrobacterial characteristics of plant seeds i-'Sing bioinoculant (Smith, 1992). New approach in invitro growth studies has been introduced as culture cotton method which are not been used previously.