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Genetic diversity within the genus Solanum (Solanaceae) as revealed by RAPD markers

机译:RAPD标记揭示茄科(茄科)内的遗传多样性

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Random amplified polymorphic DNA (RAPD) technique was used as a tool for assessing genetic diversity and species relationships among 28 accessions of eggplant representing five species. Twenty-eight samples of egg-plants were collected from differentparts of the country. A total of 144 polymorphic amplified products were obtained from 14 decamer primers, which discriminated all the accessions. The value of Jaccard’s coefficient ranged from 0.05 to 0.82. The similarity result indicates presence of high level of genetic diversity in eggplants and a dendrogram constructed by UPGMA method shows that S. incanum is closest to S. melongena followed by S. nigrum. Only one accession of S. nigrum and S. surattense was taken in the present study that showedgrouping with each other. Genetically distinct genotypes identified using RAPD markers could be potential sources of germplasm for eggplant improvement.
机译:随机扩增多态性DNA(RAPD)技术被用作评估代表五个物种的28种茄子之间遗传多样性和物种关系的工具。从该国不同地区收集了28种茄子样品。从14个decamer引物中获得了144个多态性扩增产物,这些引物区分了所有种质。 Jaccard系数的值介于0.05到0.82之间。相似性结果表明茄子中存在高水平的遗传多样性,并且通过UPGMA方法构建的树状图显示,S。incanum最接近S. melongena,其次是S. nigrum。在本研究中仅摄取了黑色链霉菌和表面链球菌的一种加入,表明彼此分组。使用RAPD标记鉴定的遗传上不同的基因型可能是茄子改良的潜在种质来源。

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