Analysis of phylogenetic relationships in the genus Solanum (Solanaceae) as revealed by RAPD markers

摘要

Phylogenetic relationships and genetic variation were examined in the genus Solanum based on the random amplified polymorphic DNA (RAPD) technique. Genetic distances were estimated for 42 accessions from five subgenera [Archaesolanum, Minon (Syn. Brevantherum), Leptostemonum, Potatoe, and Solanum]. This investigation provided new information and reinforced some suggestions from previous phylogenetic studies. Analysis with random markers from the total genome clearly separated Solanum sect. Dulcamara, from the other members of Solanum subg. Potatoe, and indicated that among the analysed Solanum subgenera subg. Solanum is most closely related to it. The results suggest that Solanum sect. Dulcamara should be excluded from Solanum subg. Potatoe. The subclusters formed by S. rostratum and S. citrullifolium appear to be distinct from the subcluster formed by the two accessions of S. sisymbriifolium. This topology indicates that Solanum sect. Androceras and Solanum sect. Cryptocarpum are fairly closely related, although the data suggest that the two sections should not be maintained.