Isolation, characterization and antimicrobial activity of marine halophilic Actinopolyspora species AH1 from the west coast of India

摘要

A halophilic Actincipolyspora species AH1 was isolated from a marine sediment sample obtained from Alibag coast, Maharashtra. The strain showed good growth in medium containing 10 to 15% (w/v) NaCl and with 30 to 36degreesC temperature. Cultural properties of Actinopolyspora were studied extensively on starch casein agar and other media. Strain AH1 showed an elon-gated and circular shape with 20 to 30 spore-chain structures observed by slide culture technique and scanning electron microscopy. The morphological, biochemical and physiological characters of the isolate conformed to the characteristics of the genus Actinopolyspora, which contained only three species, viz. A. halophila, A. mortivallis and A. iraqiensis. However, the Actinopolyspora species AH1 isolated and characterized by us showed different properties compared to the known species. It showed resistance to clindamycin, vancomycin, nalixidic acid and streptomycin antibiotics. Interestingly, it showed good antibacterial activity against Staphylococcus aureus, S. epidermidis, Bacillus subtilis and antifungal activities against Aspergillus niger, A. fumigatus, A. flavus, Fusarium oxysporum, Penicillium species and Trichoderma species. This is a report of the antimicrobial activity exhibited by Actinopolyspora species AH1 isolated from the marine sediment.