Molecular characterization of specialty mushrooms of western Rajasthan, India.

摘要

Eighteen specialty mushroom germplasm accessions were collected from Udaipur (Rajasthan) and characterized using DNA fingerprinting and rRNA gene sequencing. Phylogenetic analyses based on RAPD profiles and nucleotide sequence of 5.8S rRNA gene along with its spacer regions revealed variation of inter-generic and intra-species isolates among accessions. Based on ITS sequence polymorphism, seven isolates were identified as Podaxis pistillaris, four as Phellinus igniarius, one as Gymnopilus subearlei and six as Phellorinia herculea. The similarity matrix revealed very high intra-species homology (99.5-100%) and significant inter-generic diversity (21.2-37.7%). G. subearlei and P. herculea have been discovered as new additions to the Indian basidiomycetes biodiversity..