Label-free fluorescent aptasensor berberine-based strategy for ultrasensitive detection of Hg 2+ ion

摘要

A label-free fluorescent aptasensing platform was fabricated and a simple and rapid method to detect Hg2+ion in aqueous solution was put forward by means of berberine and Hg2+ion-aptamer are as the fluorescence probe and the recognition element, respectively. Various factors including the concentration of berberine, Hg2+ion and Hg2+ion-aptamer, pH effect and the reaction time were investigated in detail. Under the optimal experimental conditions, in the sensing system, the fluorescence intensity changes displayed a calibration response for Hg2+ion in the range of 0.1?μM to 10.0?μM and the detection limit was of 7.7?nM (S/N?=?3). The fabricated label-free fluorescence aptasensor is not only conveniently but also effectively applicable used for analysis of Hg2+ion in blood serum and tap water samples and the recovery range is of 96.0%–105.7%. In brief, this study offers an easy, economical and stable assay system for detecting Hg2+ion in rough condition.