Isolation and characterization of eighteen polymorphic microsatellite DNA loci from masu salmon (Oncorhynchus masou) using next-generation sequencing and cross-species amplification

摘要

A total of 18 novel microsatellite markers in masu salmon, an important fisheries resource around the Far East, were isolated using a next-generation sequencing approach. The number of alleles and expected heterozygosity per locus ranged 5-15 and 0.55847-0.92944, respectively. Four loci also were polymorphic in three other salmonid species after cross-species amplification. These newly isolated loci will provide important information for the sustainable adaptive management of masu salmon.