NEUROVASCULAR COUPLING PROTECTS NEURONS AGAINST HYPOXIC INJURY VIA INHIBITION OF POTASSIUM CURRENTS BY GENERATION OF NITRIC OXIDE IN DIRECT NEURON AND ENDOTHELIUM COCULTURES

摘要

This study examined the effect of neuron endothelial coupling on the survival of neurons after ischemia and the possible mechanism underlying that effect. Whole-cell patch-clamp experiments were performed on cortical neurons cultured alone or directly cocultured with brain microvascular endothelial cells (BMEC). Propidium iodide (PI) and NeuN staining were performed to examine neuronal death following oxygen and glucose deprivation (OGD). We found that the neuronal transient outward potassium currents (I-A) decreased in the coculture system, whereas the outward delayed-rectifier potassium currents (I-K) did not. Sodium nitroprusside, a NO donor, enhanced BMEC-induced I-A inhibition and nitro-L-arginine methylester, a NOS inhibitor, partially prevented this inhibition. Moreover, the neurons directly cocultured with BMEC showed more resistance to OGD-induced injury compared with the neurons cultured alone, and that neuroprotective effect was abolished by treatment with NS5806, an activator of the I-A. These results indicate that vascular endothelial cells assist neurons to prevent hypoxic injury via inhibiting neuronal I-A by production of NO in the direct neuron-BMEC coculture system. These results further provide direct evidence of functional coupling between neurons and vascular endothelial cells. This study clearly demonstrates that vascular endothelial cells play beneficial roles in the pathophysiological processes of neurons after hypoxic injury, suggesting that the improvement of neurovascular coupling or functional remodeling may become an important therapeutic target for preventing brain injury. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.