Downregulation of miR-146a promotes proliferation and migration of AOB-treated embryoid body via PDGFRA induction

摘要

Antioxidant of bamboo leaves (AOB) has been proven to have antioxidant activity and an inhibitory effect on free radicals that induce deterioration of macromolecules. The multi-target regulation of microRNAs (miRs) in the complicated process of vasculogenesis and angiogenesis lead to the use of miRNA therapy in vascular development. In the present study, the role of miRNAs on early embryo vascular development upon AOB stimulation was investigated. For this purpose, mouse embryonic stem cells were spontaneously differentiated as embryoid bodies (EBs) and were examined by phase contrast microscopy. miR-146a mimic and scramble control were transfected into EBs and potential targets of miR-146a were predicted. Cell proliferation and migration were detected by cell viability and wound-healing and migration assays, respectively. Angiogenesis was determined by the Spheroid sprouting assay. It was demonstrated that EBs transfected with miR-146a mimic had an increased growth rate compared with the control cells. miR-146a-transfected cells were very susceptible to AOB treatment. Furthermore, among the predicted miR-146a targets, platelet-derived growth factor receptor alpha (PDGFRA) was identified as a bona fide target of miR-146a. In conclusion, PDGFRA was demonstrated to participate in the modulation of cell migration and proliferation of mouse EBs. The present study expanded the current understanding of AOB biology and elucidated the mechanisms underlying early embryo vascular development upon AOB stimulation.